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| Study on Mutagenesis, Bacteriostasis and Disease Prevention Effect of Bacillus velezensis ZF145 |
| LIU Shi-Cheng1,2,*, LI Lei2,*, ZHANG Xue-Yan3, SHI Yan-Xia2, CHAI A-Li2, FAN Teng-Fei2, LI Bao-Ju2,**, XIE Xue-Wen2,** |
1 College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China;
2 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
3 School of Agricultural, Ningxia University, Yinchuan 750021, China |
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Abstract Bacterial angular spot of cucumber is one of the common bacterial diseases in the greenhouse cucumber (Cucumis sativus) cultivation. Using biocontrol bacteria is an important method to control the disease. In order to improve the antagonistic activity of Bacillus velezensis against bacterial angular spot of cucumber, the methods of ultraviolet (UV) mutagenesis and the atmospheric room temperature plasma (ARTP) mutagenesis were used to mutagenize the Bacillus velezensis strain ZF145. Taking Pseudomonas amygdali pv. Lachrymans as the indicator bacteria, screening was carried out by plate confrontation and in vivo experiment, and the mutation efficiency of the 2 mutagenesis methods was compared. The results showed that the control effect of positive mutant strain A561 on cucumber bacterial angular spot was 63.61%, which was much higher than that of ZF145 and the 5% Zhongshengmycin Wettable Powder. The expressions of the surfactin synthetase gene clusters (srf) genes srfAA, srfAB and srfAC associated with the synthesis of surfactin were significantly up-regulated (P<0.05). The positive mutation rate of ARTP mutation was 10.86%, much higher than that of UV mutation, which was more suitable for mutation breeding of Bacillus. This study provides a theoretical support for the mutation breeding of Bacillus velezensis, and is of great significance for the biological control of bacterial angular spot of cucumber.
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Received: 24 February 2022
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Corresponding Authors:
** libaoju@caas.cn; xiexuewen@caas.cn
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| About author:: * These authors contributed equally to this work |
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