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| Construction of Porcine (Sus scrofa) Induced Pluripotent Stem Cell Lines with Over-expression of CD163 |
| YUE Wei, ZHANG Ju-Qing, YANG Xin-Chun, WU Xiao-Long, SHEN Qiao-Yan, YU Shuai, ZHU Zheng-Shuo, WANG Cheng-Bao, ZHANG Shi-Qiang, HUA Jin-Lian* |
| Shaanxi Stem Cell Engineering and Technology Research Center/College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China |
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Abstract CD163 is a macrophage-specific protein in the cysteine superfamily rich in scavenger receptors and is the most important cellular receptor of Porcine reproductive and respiratory syndrome virus (PRRSV). Induced pluripotent stem cells (iPSCs) are pluripotent stem cells with biological characteristics of embryonic stem cells obtained by reprogramming after introducing foreign transcription factors into somatic cells. Firstly, porcine (Sus scrofa) CD163 gene was transferred into porcine induced pluripotent stem cells (piPSCs) by lentiviral vector system, and an induced pluripotent stem cell line stably expressing CD163 was established, which was named CD163OE-piPSC. The susceptibility of the cell line to PRRSV was verified by virus infection, and the pluripotency and proliferation of iPSCs were not affected by alkaline phosphatase staining, cell population doubling time test and 5-ethynyl-2'- deoxyuridine (EdU) cell proliferation test.The cell lines obtained in this study could be effectively used to study the pathogenesis of PRRSV-host interaction and screen the target molecules of viral diseases.
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Received: 19 October 2021
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Corresponding Authors:
* jinlianhua@nwsuaf.edu.cn
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