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| Study on Polyamine Content and Expression of Polyamine Metabolism Genes in Brain Tissues from Nonghua Ducks (Anas platyrhynchos) |
| KANG Li-Juan, ZHOU Xue-Min, DAN Hong-Ying, GE Fan-Zi, LI Shuo, WANG Xin, WANG Ze-Long, NIU Chun-Yang, GUO Yong-Ni, KANG Bo* |
| College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611100, China |
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Abstract Polyamines are involved in regulating the development and regeneration of nervous system.To study the developmental changes of polyamine metabolism in cerebrum and cerebellum tissues from Nonghua ducks (Anas platyrhynchos) at different day-age, polyamine content in cerebrum and cerebellum tissues from ducks at 0~120 day-age were determined by HPLC. The relative abundance of genes related to polyamine metabolism were determined by qPCR. The result showed that contents of spermidine and spermine in cerebrum and cerebellum tissues were significantly higher than putrescine (P<0.05), and the contents of polyamine decreased with the increase of day-age. Putrescine content (30.80 mg/kg) in cerebrum tissues at 60 day-age was significantly higher than others (P<0.05). Spermidine content (643.26 mg/kg) in cerebrum tissues at 0 day-age was significantly higher than others (P<0.05). Spermine content in cerebrum tissues at 0 day-age (1665.95 mg/kg) and 120 day-age (1623.66 mg/kg) were significantly higher than others (P<0.05). Spermidine (54.09 mg/kg) and spermine (413.5 mg/kg) contents in cerebellum tissues at 0 day-age were significantly higher than others (P<0.05). Putrescine content in cerebellum tissues at 0 day-age (26.46 mg/kg) and 120 day-age (22.03 mg/kg) were significantly higher than others (P<0.05). The expression levels of key genes related to polyamine metabolism decreased in cerebrum tissues of ducks at 30 day-age, increased at 60 day-age, decreased at 90 day-age, and then increased at 120 day-age. The expression levels of key genes related to polyamine metabolism in cerebellum tissues of ducks at 0 day-age were relatively higher than that of other ducks. The trend in gene expression levels was different. This study reveals the changes of polyamine content and the expression levels of key genes related to polyamine metabolism in brain tissues from different age ducks, and provides basic data for polyamine regulating brain growth and development in ducks.
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Received: 01 November 2021
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Corresponding Authors:
* bokang@sicau.edu.cn
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