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Screening and Analysis of RNA Methyltransferase From Plant Pathogenic Fungi |
JIA Ming-Xuan1, ZHOU He1, WANG Mao-Cun1, SUN Han-Di1, CHENG Zhen1, FAN Yong-Shan2, GONG Xiao-Dong1, GU Shou-Qin1, LIU Yu-Wei1,*, DONG Jin-Gao1,* |
1 College of Life Sciences, Hebei Agricultural University/Key Laboratory of Plant Physiology and Molecular Pathology of Hebei Province/State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, China; 2 Department of Life Sciences, Tangshan Normal University, Tangshan 063002, China |
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Abstract RNA methylation is the most important form of RNA modification, and N6-methyladenosine (m6A) is the most prevalent post-transcriptional modification of eukaryotic mRNA, which plays an important role in the regulation of growth and development of organisms. RNA methyltransferase acts as the major regulators of RNA methylation with few documented functional dissections focused on phytopathogenic fungi of plants. In the present study, structure and function of RNA methyltransferase in plant pathogenic fungi were explored via bioinformatics methods. The results showed that a total of 159 RNA methyltransferase genes were identified in 106 phytopathogenic fungi. The analysis of physicochemical properties showed that the average length of amino acids (aa) encoded by 159 phytopathogenic fungi RNA methyltransferases was about 470 aa (ranging from 179 to 1206 aa), the average molecular weight was about 52.64 kD (variation range 20.04~134.35 kD), and the average isoelectric point was about 7.26 (variation range 4.90~10.28). The evolutionary relationship of phytopathogenic fungi RNA methyltransferases was relatively conservative. The RNA methyltransferases which in the same phylum were generally clustered in the same clade, and the conservation motifs in same clade had highly similarity. Furthermore, Real-time qPCR method was used to analyze the expression pattern of gene encoding RNA methyltransferases in Setosphaeria turcica (StMETTL1) at different developmental stages. It was found that the expression level of StMETTL1 was significantly increased in infected nails stage (P<0.05), suggesting that StMETTL1 may play an important role in the process of S. turcica infection of the host. This study provides data support for further understanding the role of m6A in plant pathogenic fungi.
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Received: 11 January 2022
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Corresponding Authors:
*liuyw@hebau.edu.cn;dongjingao@126.com
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[1] Adams J M, Cory S.1975. Modified nucleosides and bizarre 5′-termini in mouse myeloma mRNA[J]. Nature, 255(5503): 28-33. [2] Avery S V, Singleton I, Magan N, et al.2019. The fungal threat to global food security[J]. Fungal Biology, 123(8): 555-557. [3] Beemon K, Keith J.1977. Localization of N6-methyladenosine in the Rous sarcoma virus genome[J]. Journal of Molecular Biology, 113(1): 165-179. [4] Bodi Z, Zhong S, Mehra S, et al.2012. Adenosine methylation in Arabidopsis mRNA is associated with the 3′ end and reduced levels cause developmental defects[J]. Frontiers in Plant Science, 3: 48. [5] Bokar J A, Rath-Shambaugh M E, Ludwiczak R, et al.1994. Characterization and partial purification of mRNA N6-adenosine methyltransferase from HeLa cell nuclei. Internal mRNA methylation requires a multisubunit complex[J]. Journal of Biological Chemistry, 269(26): 17697-17704. [6] Bujnicki J M, Feder M, Radlinska M, et al.2002. Structure prediction and phylogenetic analysis of a functionally diverse family of proteins homologous to the MT-A70 subunit of the human mRNA: M6A methyltransferase[J]. Journal of Molecular Evolution, 55(4): 431-444. [7] Chen-Kiang S, Nevins J R, Darnell Jr J E.1979. N6-methyl-adenosine in Adenovirus type 2 nuclear RNA is conserved in the formation of messenger RNA[J]. Journal of Molecular Biology, 135(3): 733-752. [8] Clancy M J, Shambaugh M E, Timpte C S, et al.2002. Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: A potential mechanism for the activity of the IME4 gene[J]. Nucleic Acids Research, 30(20): 4509-4518. [9] Desrosiers R C, Friderici K H, Rottman F M.1975. Characterization of Novikoff hepatoma mRNA methylation and heterogeneity in the methylated 5' terminus[J]. Biochemistry, 14(20): 4367-4374. [10] Duan H C, Wei L H, Zhang C, et al.2017. ALKBH10B is an RNA N6-methyladenosine demethylase affecting Arabidopsis floral transition[J]. Plant Cell, 29(12): 2995-3011. [11] Feng J, Zhang T, Sorel O, et al.2022. Global profiling reveals common and distinct N6-methyladenosine (m6A) regulation of innate immune responses during bacterial and viral infections[J]. Cell Death & Disease. 13(3): 234. [12] Frye M, Harada B T, Behm M, et al.2018. RNA modifications modulate gene expression during development[J]. Science, 361(6409): 1346-1349. [13] Guil S, Esteller M.2015. RNA-RNA interactions in gene regulation: The coding and noncoding players[J]. Trends in Biochemical Sciences, 40(5): 248-256. [14] Hu J, Cai J, Umme A, et al.2022. Unique features of mRNA m6A methylomes during expansion of tomato (Solanum lycopersicum) fruits[J]. Plant Physiology, 188(4): 2215-2227. [15] Knogge W.1996. Fungal infection of plants[J]. Plant Cell, 8(10): 1711-1722. [16] He L, Li H, Wu A, et al.2019. Functions of N6-methyladenosine and its role in cancer[J]. Molecular Cancer. 18: 176. [17] Lu L, Zhang Y, He Q Z, et al.2020. MTA, an RNA m(6)A methyltransferase, enhances drought tolerance by regulating the development of trichomes and roots in poplar[J]. International Journal of Molecular Sciences, 21(7): 2462. [18] Moss B, Gershowitz A, Stringer J R, et al.1977. 5'-terminal and internal methylated nucleosides in herpes simplex virus type 1 mRNA[J]. Journal of Virology, 23(2): 234-239. [19] Perry R P, Kelley D E, Friderici K, et al.1975. The methylated constituents of l cell messenger RNA: Evidence for an unusual cluster at the 5′ terminus[J]. Cell, 4(4): 387-394. [20] Ping X L, Sun B F, Wang L, et al.2014. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase[J]. Cell Research, 24(2): 177-189. [21] Růžička K, Zhang M, Campilho A, et al.2017. Identification of factors required for m(6)A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI[J]. New Phytologist, 215(1): 157-172. [22] Scutenaire J, Deragon, J M., Jean V., et al.2018. The YTH domain protein ECT2 is an m(6)A reader required for normal trichome branching in Arabidopsis[J]. Plant Cell, 30(5): 986-1005. [23] Shen H, Luo B, Wang Y, et al.2022. Genome-wide identification, classification and expression analysis of m(6)A gene family in Solanum lycopersicum[J]. International Journal of Molecular Sciences. 23(9): 4522. [24] Shen L, Liang Z, Gu X, et al.2016. N6-methyladenosine RNA modification regulates shoot stem cell fate in Arabidopsis[J]. Developmental Cell. 38(2): 186-200. [25] Tuck M T.1992. Partial purification of a 6-methyladenine mRNA methyltransferase which modifies internal adenine residues[J]. Biochemical Journal, 288(1): 233-240. [26] Yang D, Xu H, Liu Y, et al.2021. RNA N6-methyladenosine responds to low-temperature stress in tomato anthers[J]. Frontiers in Plant Science. 12: 687826. [27] Yang Y, Hsu P J, Chen Y S, et al.2018. Dynamic transcriptomic m6A decoration: Writers, erasers, readers and functions in RNA metabolism[J]. Cell Research, 28(6): 616-624. [28] Yen Y P, Chen J A.2021. The m6A epitranscriptome on neural development and degeneration[J]. Journal of Biomedical Science. 28: 40. [29] Yue H, Nie X, Yan Z, et al.2019. N6-methyladenosine regulatory machinery in plants: Composition, function and evolution[J]. Plant Biotechnology Journal. 17(7): 1194-1208. [30] Zhang F, Zhang Y C, Liao J Y, et al.2019. The subunit of RNA N6-methyladenosine methyltransferase OsFIP regulates early degeneration of microspores in rice[J]. PLoS Genetics, 15(5): e1008120. [31] Zhong S, Li H, Bodi Z, et al.2008. MTA is an Arabidopsis messenger RNA adenosine methylase and interacts with a homolog of a sex-specific splicing factor[J]. Plant Cell, 2008, 20(5): 1278-1288. [32] Zhou L, Gao G, Tang R, et al.2022. m6A-mediated regulation of crop development and stress responses[J]. Plant Biotechnology Journal, https://doi.org/10.1111/mpp.13239. [33] Zhu C, Zhang S, Zhou C, et al.2021. Genome-wide investigation of N6-methyladenosine regulatory genes and their roles in tea (Camellia sinensis) leaves during withering process[J]. Frontiers in Plant Science. 12: 702303. |
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