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| Cloning, Tissue Distribution of mstn and the Effects of Starvation and Re-feeding on the Expression of mstn in Muscle of Acipenser dabryanus |
| WU Xiao-Yun, CHEN Ye-Yu, LAI Jian-Sheng, LIU Ya, SONG Ming-Jiang, LI Fei-Yang, GONG Quan* |
| Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu 611731, China |
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Abstract Myostatin (mstn) is a negative regulator of muscle growth and belongs to transforming growth factor β (TGF-β family members), which play a role by inhibiting myoblast proliferation. In order to study the mechanism of mstn in the compensatory growth of Acipenser dabryanus, the CDS region of mstn was cloned according to the results of previous transcriptome sequencing, and the expression of mstn in various tissues and starvation and refeeding conditions was analyzed. The results showed that the total length of mstn (GenBank No. MZ666180) was 2 552 bp, and the CDS region was 1 122 bp, encoding 373 amino acids. The highest expression was in muscle, followed by skin, gill and eye. Under starvation condition, the expression of mstn in muscle of A. dabryanus gradually decreased with the prolongation of starvation time; after 14 d of refeeding, the expression of mstn in 3 d group was significantly higher than that in control group (P<0.05), and there was no significant difference between 7 or 14 d groups and control group (P>0.05). The results showed that starvation could inhibit mstn transcription of A. dabryanus. It showed that starvation could inhibit the transcription of mstn in A. dabryanus, and supplement of nutrients after short-term starvation could activate the muscle mstn function of A. dabryanus. It provides a reference for evaluating the physiological adaptation mechanism of A. dabryanus to environmental stress, and helps us to understand the growth and growth regulation mechanism of A. dabryanus, and provides a reference for future production practice.
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Received: 25 April 2021
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Corresponding Authors:
*admiral671@163.com
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