EMS诱变创制小麦叶锈病菌致病性变异菌株

doi:10.3969/j.issn.1674-7968.2021.11.019

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Abstract Wheat leaf rust is one of the most serious diseases of wheat (Triticum aestivum). It is of great significance to create single-base mutants of Puccinia triticina (Pt) for the study on virulence genes and pathogenic mechanisms. Therefore, ethyl methyl sulfonate (EMS) was used to mutate the Pt. It was effective to inhibit the germination of Pt urediniospore by treating with 0.04 mol/L EMS for 8 min, and the inhibition rate reached 83.69%. Nineteen mutant isolates of 2008-5-9-2 (KHHT), 2013-5-72-1 (THSN), 2013-5-28-1 (JHKT) and 2017-8-1465 (PHST) induced by EMS were obtained, and the mutant rate was 95%. The infection types of the mutant strains were detected on 18 wheat leaf rust resistance near-isogenic lines (cultivars). It was found that the infection types of the mutants changed on 15 near-isogenic lines. Strain Mu2008-5-9-2② was virulent to leaf rust resistance gene 24 (Lr24) and strains Mu2013-5-28-1④, Mu2008-5-9-2②, Mu2008-5-9-2④, Mu2008-5-9-2⑤, Mu2013-5-28-1③, Mu2013-5-28-1⑥, Mu2013-5-28-1⑦, Mu2013-5-28-1① and Mu2013-5-28-1② were avirulent to Lr2a, Lr14a, Lr16, Lr17 and Lr33. The results provide basic materials for the analysis of mutant loci in leaf rust and the cloning of avriulence gene of Lr24, Lr2a, Lr14a, Lr16, Lr17 and Lr33 in future.

Key words： Puccinia triticina (Pt) Chemical mutation Ethyl methyl sulfonate (EMS) Virulence variation

Received: 20 January 2021

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S512.1

Corresponding Authors: * wenxiangyang2003@163.com

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	WEI Jie
	ZHOU Zong-Yue
	QI Yue
	ZHANG Na
	MENG Qing-Fang
	YANG Wen-Xiang

Cite this article:

WEI Jie,ZHOU Zong-Yue,QI Yue, et al. Creation of Pathogenic Mutant Strains of Puccinia triticina by EMS Mutation[J]. 农业生物技术学报, 2021, 29(11): 2259-2266.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.11.019 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I11/2259

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