LAMP扩增产物检测方法研究进展及基因编辑技术在其中的应用

doi:10.3969/j.issn.1674-7968.2021.10.016

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Abstract With the rapid development of isothermalamplification technology, the method to detect amplicons has become the key restricting its rapid popularization and application. Given the characteristics of Loop-mediated isothermal amplification(LAMP) reaction and the current amplicons detection methods, we summarized the progresses which have been made in recent years, including methods based on by-product generation analysis, methods aiming for multiplex detection, and the sequence-specific ones with visual readouts (e.g. CRISPR/Cas based ones). The possible opportunities and challenges are also been discussed. This review provides support for the nucleic acid analysis industry and applications based on nucleic acid amplification.

Key words： Loop-mediated isothermal amplification (LAMP) Amplicons detection Nucleic acid Isothermal amplification

Received: 01 February 2021

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Corresponding Authors: * fangzh921@fzu.edu.cn

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	BAI Rong
	BAI Lin-Lin
	WANG Shao-Yun
	ZHANG Fang

Cite this article:

BAI Rong,BAI Lin-Lin,WANG Shao-Yun, et al. Research Progress of LAMP Amplicons Detection Method and the Application of Gene Editing Technology[J]. 农业生物技术学报, 2021, 29(10): 2016-2030.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.10.016 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I10/2016

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