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| Identification of JAZ Gene Family in Cajanus cajan and Expression Analysis in Response to Pathogenic Fungus Cc1-1 |
| LI Na1, SONG Zhi -Hua1, FAN Yu-Xin1, DONG Bi-Ying1, CAO Hong-Yan1, DU Ting-Ting1, LIU Teng-Yue1, YANG Wan-Long1, YANG Qing1,2, MENG Dong1,2,*, FU Yu-Jie1,2,* |
1 College of Forestry, Beijing Forestry University, Beijing 100083, China;
2 Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China |
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Abstract Jasmonates play a key role in plant response to biological stresses, and jasmonate ZIM-domain (JAZ) is a critical factor in jasmonic acid (JA) signal pathway. The present study screened out 24 members of JAZ family in pigeonpea (Cajanus cajan) genome, and explored phylogenetic analysis, gene chromosome mapping and protein conserved domain analysis. According to previous transcriptome data, the expression patterns of JAZs in roots and leaves were analyzed, and the expression of JAZs in leaves in response to pathogenic fungus Cc1-1 was detected by reverse transcription-PCR (RT-PCR). The results showed that there were 129~376 amino acid residues in JAZ family, and the isoelectric points were 5.94~9.37. JAZ family members could be divided into 5 subfamilies by MEGA software, and they were unevenly distributed on 6 chromosomes. The subcellular localization prediction showed that CcJAZ12 and CcJAZ18 were located in the chloroplast and CcJAZ19 in the cytoskeletal, and all the other 21 JAZs were located in the nucleus. The prediction results of cis-acting elements in the promoters showed that JAZ family genes had certain amount of JA responsive elements, and a large number of light responsive elements and a small number of abscisic acid, salicylic acid and other hormone regulatory elements. Transcriptome analysis showed that the expression of JAZs in leaves was higher than that in roots, and the expression of CcJAZ19 was the highest, which might play a more important role in the response of leaves to biological stresses. After infection of pigeonpea leaves by pathogenic fungus Cc1-1, the gene expression of JAZs in the leaves increased gradually with the infection time, and the expression of CcJAZ8, CcJAZ19, and CcJAZ23 was significantly up-regulated. The expression level of CcJAZ19 was up-regulated to 2.5 times (P<0.05) at 6 h after infection, indicating that it might participate in the defense response of JA signal to fungi. The present study provides basic data for the follow-up study on molecular regulation mechanism of JA signal.
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Received: 04 February 2021
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Corresponding Authors:
* mengdongjlf@163.com; yujiefu67@163.com
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