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| Responses of PheFT6 and PheFT17 Genes in Phyllostachys pubescens to External Environment and Protein Interaction Analysis |
| LIU Li, CHEN Jiao-Yu, SHAO Ming-Xia, LIU Shen-Kui, GUO Xiao-Qin* |
| State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China |
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Abstract Moso bamboo (Phyllostachys edulis) is an important economical bamboo species in China. Shoot bud germination and development is one of the main factors limiting its productivity. The phosphatidyl ethanolamine-binding protein (PEBP) family contains FLOWERING LOCUS T (FT) subfamily, TERMINAL FLOWER 1 (TFL1) subfamily and MOTHER OF FT AND TFL1 (MFT) subfamily. The FT subfamily plays an important role in the growth and development of plants. In order to explore the function of FT gene in the process of shoot bud development, 2 FT homologous genes, PheFT6 and PheFT17, were cloned in Ph. edulis, and their expression patterns were analyzed by qRT-PCR, and their interaction proteins were explored by yeast two-hybrid. The results showed that the lengths of the coding regions of PheFT6 (GenBank No. MT976159) and PheFT17 (GenBank No. MT976160) were 525 and 528 bp, encoding 174 and 175 amino acids, respectively, which contained the PEBP domain. The PheFT6 gene typically contains 4 exons and 3 introns, while the PheFT17 gene has additional 2 exons inserted, resulting in alternative splicing that produce at least 4 transcripts. Phylogenetic tree analysis showed that PheFT6 and PheFT17 were far from rice Heading date 3a (Hd3a) and Arabidopsis thaliana FT. qRT-PCR analysis showed that the expression of PheFT6 and PheFT17 genes showed obvious circadian rhythm, with low expression level in the daytime and high expression level at night. The expression of PheFT6 gene was induced by low temperature (12 ℃), while the expression of PheFT17 gene was induced by warm temperature (28 ℃). Compared with the seedlings cultured at 12 ℃, the seedlings treated at 28 ℃ had higher tillering ability. The expression of PheFT6 and PheFT17 genes was strongly inhibited by drought, and the inhibition increased with the increasing of drought degree. The yeast two-hybrid results showed that both PheFT6 and PheFT17 did not interact with PheGF14b and PheGF14c in 14-3-3 (G-box factor 14-3-3 protein), but interacted with PheTB1L-1 and PheTB1L-2, homologues of Hub protein BRANCHED1(BRC1)/TEOSINTE BRANCHED1 (TB1), during branching, which suggested that PheFT6 and PheFT17 might be involved in branching and tillering in Ph. edulis. This study provides a reference for further analysis of the biological functions of PheFT6 and PheFT17, and could provide basic data for the study on molecular mechanism of shoot development.
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Received: 28 July 2020
Published: 01 March 2021
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Corresponding Authors:
*xqguo@zafu.edu.cn
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