玉米皱叶突变体<em>wl1</em>表型分析与基因定位

doi:10.3969/j.issn.1674-7968.2021.03.001

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Abstract Leaf shape is one of the important agronomic traits of maize. Moderate leaf shape is of great significance to increase maize planting density and yield. Leaf shape is regulated by multiple genes and genetic loci. However, the molecular mechanism of leaf morphological development in maize is still unclear. In this study, wrinkled leaf 1 (wl1) which was a leaf shrinkage mutant, was obtained by treating pollen of maize inbred line Mo17 with ethyl methanesulfonate. Genetic analysis showed wl1 was a recessive mutant controlled by a single gene. wl1 showed that small ridges and trichomes on the leaf surface, and the number of stomata decreased compared with wildtype. Paraffin section showed that the epidermal cell layers on the wl1 leaf was doubled. Besides, maize plant height, length and width of ear leaf, the first and second leaf above ear leaf were decreased compared with wildtype (P<0.01). Transcriptome sequencing analysis indicated the expression of several genes involved in photosynthesis relative biological processes and cellular component were decreased in wl1. Meanwhile, photosynthetic measurement showed the net assimilation rate, stomatal conductance and transpiration rate of wl1 were significantly lower than wild type (P<0.01). wl1 was located on chromosome 1 within the interval of physical distance of 1.7 Mb by using map-based cloning. According to analyzing the expression level of candidate genes in interval, genome resequencing and sequence alignment with seven different maize inbred lines, the candidate gene of wl1 was preliminarily identified as Zm00001d027266, which encoded a DeSI (desumoylating isopeptidase)-like protein. This study lays the foundation for the cloning of wl1 gene and the molecular mechanism analysis of maize leaf shape development.

Key words： Maize wl1 (wrinkled leaf 1) Transcriptome analysis Gene mapping

Received: 05 July 2020 Published: 01 March 2021

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Corresponding Authors: **caiyilin@163.com
*The authors who contribute equally

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	ZHOU Lian
	BAI Yang
	DONG Er-Fei
	Qin Li-Ping
	JING Xiao-Jie
	ZHU Ting-Ting
	TIAN Miao
	WANG Ting
	LIU Chao-Xian
	CAI Yi-Lin

Cite this article:

ZHOU Lian,BAI Yang,DONG Er-Fei, et al. Phenotype Analysis and Gene Mapping of Maize (Zea mays) Wrinkled Leaf Mutant wl1 (wrinkled leaf 1)[J]. 农业生物技术学报, 2021, 29(3): 413-423.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.03.001 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I3/413

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