基于KASP技术的牛3种无角基因联合检测方法研究

doi:10.3969/j.issn.1674-7968.2021.03.020

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Abstract In wild animals, horns are used to fight for survival and mates. However, in the intensive farming system, polledness is a favorable trait because helps avoid the cost and protect animal welfare. Polledness is an autosomal dominant trait with multiple variants/alleles. Three types of polled variants have been reported on chromosome 1 of taurine cattle (Bos taurus) - the Friesian variant that occurred in dairy cattle breeds, the Celtic variant that was found in beef and dual-purposed breeds, and the Mongolian variant that was identified in Mongolian cattle. In this study, based on a genotyping platform that combines KASP (kompetitive allele-specific PCR) assay and microfluidic chip, a molecular diagnostic method was developed to detect the three polled variants simultaneously. The new KASP assay was further validated by using the conventional molecular experiments including PCR amplification, gel electrophoresis and Sanger sequencing, demonstrating that the new method was accurate and reliable and could be applied in large-scale testing. Interestingly, the Mongolian polled variant was detected in a bull of Sanhe cattle, an indigenous Chinese cattle breed. In addition, a previously unpublished deletion of 2 bp was identified in the Friesian variant that could be used as a marker associated with polledness. These results provide a technical basis for molecular breeding for polled cattle.

Key words： Kompetitive allele-specific PCR (KASP) Polled gene Sanhe cattle Molecular assay Microfluidic chip

Received: 04 August 2020 Published: 01 March 2021

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S823

Corresponding Authors: *yizhang@cau.edu.cn

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	Articles by authors
	YAN Ze
	XIAO Wei
	ZHANG Sheng-Li
	WANG Ya-Chun
	ZHANG Yi

Cite this article:

YAN Ze,XIAO Wei,ZHANG Sheng-Li, et al. Multiplex Detection of Three Cattle (Bos taurus) Polled Variants Based on KASP Assay[J]. 农业生物技术学报, 2021, 29(3): 610-618.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.03.020 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I3/610

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