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| Correlation Analysis Between Heterosis Performances, Gene Expression at Seeding Stage and Yield of Hybrids Rice (Oryza sativa) |
| WANG Ying-Heng1,2, Xu Jing3, CAI Qiu-Hua1,2, LIN Qiang1,2, HE Wei1,2, XIE Hong-Guang1,2, XIE Hua-An1,2, ZHANG Jian-Fu1,2,* |
1 Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China;
2 Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural, P. R. China/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology/Base of South-China, National Key Laboratory of Hybrid Rice/National Rice Engineering Laboratory of China, Fuzhou 350003, China;
3 Institute of Cereal Crops, Hainan Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Breeding of Hainan Province, Haikou 571100 ,China |
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Abstract Plants often show significant heterosis at the early developmental stage, and the seedling stage is the most important stage for rice (Oryza sativa) architectural development and yielding ability. In order to investigate the relationship between heterosis performance as well as genes expression levels of hybrid rice at seedling stage and the heterosis of yield in field, and to explore the possibility of yield potential prediction for hybrid rice with their performance at seedling stage, 18 representative hybrid parental lines were obtained to make an incomplete diallel crosses, and 45 hybrids rice were obtained. Heterosis performance of root, shoot, and genes expression pattern at the seedling stage, as well as the relationship between yield and traits mentioned were systematically analyzed. The correlated relationship was detected between the above-ground and underground traits at the same seedling stage, as well as traits in two different periods for the same tissue. 12 pairs of comparison between the trait value were significantly correlated, and two seedling traits were positively correlated with yield (P<0.05). Middle parent heterosis (MPH) heterosis at the early stage of seedling (3DAS) was higher than any other stages. 13 pairs of comparison between MPH values of seedling traits were positively correlated. Root length (RL)-3DAS, RL-14DAS and plant height (PH)-3DAS were correlated with the MPH value of yield (P<0.05). In addition, the expression patterns of the 4 root development related genes were observed at 17 DAS among hybrids and parental lines. Three of them were correlated with the dry root weight and shoot at 17 DAS, and all of them were correlated with the yield in the field (P<0.05). High level of heterosis performances were detected at early seedling stage. The correlations of heterosis value among traits at seedling stage were ubiquitous. Some seedling traits and the expression level of root related genes in hybrids at seedling stage were significantly correlated with the yield of hybrids. This study shed light on the model for heterosis prediction based on the early traits of hybrid rice and also provides a theoretical basis for hybrid rice breeding.
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Received: 14 May 2020
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Corresponding Authors:
*jianfzhang@163.com
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