Correlation Analysis Between Heterosis Performances, Gene Expression at Seeding Stage and Yield of Hybrids Rice (Oryza sativa)
WANG Ying-Heng1,2, Xu Jing3, CAI Qiu-Hua1,2, LIN Qiang1,2, HE Wei1,2, XIE Hong-Guang1,2, XIE Hua-An1,2, ZHANG Jian-Fu1,2,*
1 Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China; 2 Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural, P. R. China/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology/Base of South-China, National Key Laboratory of Hybrid Rice/National Rice Engineering Laboratory of China, Fuzhou 350003, China; 3 Institute of Cereal Crops, Hainan Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Breeding of Hainan Province, Haikou 571100 ,China
Abstract Plants often show significant heterosis at the early developmental stage, and the seedling stage is the most important stage for rice (Oryza sativa) architectural development and yielding ability. In order to investigate the relationship between heterosis performance as well as genes expression levels of hybrid rice at seedling stage and the heterosis of yield in field, and to explore the possibility of yield potential prediction for hybrid rice with their performance at seedling stage, 18 representative hybrid parental lines were obtained to make an incomplete diallel crosses, and 45 hybrids rice were obtained. Heterosis performance of root, shoot, and genes expression pattern at the seedling stage, as well as the relationship between yield and traits mentioned were systematically analyzed. The correlated relationship was detected between the above-ground and underground traits at the same seedling stage, as well as traits in two different periods for the same tissue. 12 pairs of comparison between the trait value were significantly correlated, and two seedling traits were positively correlated with yield (P<0.05). Middle parent heterosis (MPH) heterosis at the early stage of seedling (3DAS) was higher than any other stages. 13 pairs of comparison between MPH values of seedling traits were positively correlated. Root length (RL)-3DAS, RL-14DAS and plant height (PH)-3DAS were correlated with the MPH value of yield (P<0.05). In addition, the expression patterns of the 4 root development related genes were observed at 17 DAS among hybrids and parental lines. Three of them were correlated with the dry root weight and shoot at 17 DAS, and all of them were correlated with the yield in the field (P<0.05). High level of heterosis performances were detected at early seedling stage. The correlations of heterosis value among traits at seedling stage were ubiquitous. Some seedling traits and the expression level of root related genes in hybrids at seedling stage were significantly correlated with the yield of hybrids. This study shed light on the model for heterosis prediction based on the early traits of hybrid rice and also provides a theoretical basis for hybrid rice breeding.
WANG Ying-Heng,Xu Jing,CAI Qiu-Hua, et al. Correlation Analysis Between Heterosis Performances, Gene Expression at Seeding Stage and Yield of Hybrids Rice (Oryza sativa)[J]. 农业生物技术学报, 2021, 29(1): 23-34.
[1] Baldauf J A, Marcon C, Paschold A, et al.2016. Nonsyntenic genes drive tissue-specific dynamics of differential, nonadditive, and allelic expression patterns in maize hybrids[J]. Plant Physiology, 171(2): 1144-1155. [2] Benková E, Ivanchenko M.G, Friml, J, et al.2009. A morphogenetic trigger: Is there an emerging concept in plant developmental biology[J]. Trends in Plant Science, 14(4): 189-193. [3] Bruce A B.1910. The mendelian theory of heredity and the augmentation of vigor[J]. Science, 32(827): 627-628. [4] Coudert Y, Périn C, Courtois X, et al.2010. Genetic control of root development in rice, the model cereal[J]. Trends in Plant Science, 15(4): 219-226. [5] Dan Z, Hu J, Zhou W, et al.2015. Hierarchical additive effects on heterosis in rice (Oryza sativa L.)[J]. Frontiers in Plant Science, 6: 738-748 [6] Ding H, Qin C, Luo X, et al.2014. Heterosis in early maize ear inflorescence development: A genome-wide transcription analysis for two maize inbred lines and their hybrid[J]. International Journal of Molecular Sciences, 15(8): 13892-13915. [7] East E M, Jones D F.1920. Inbreeding and outbreeding: Their genetic and sociological significance[J]. Science, 23(1321): 415-417. [8] Groszmann M, Gonzalez-Bayon R, Greaves lK, et al.2014. Intraspecific Arabidopsis hybrids show different patterns of heterosis despite the close relatedness of the parental genomes[J]. Plant Physiology, 166(1): 265-280. [9] Guo M, Ruan W, Li C, et al.2015. Integrative comparison of the role of the phosphate response1 subfamily in phosphate signaling and homeostasis in rice[J]. Plant Physiology, 168(4): 1762-1776. [10] Herbst R H, Bar-Zvi D, Reikhav S, et al.2017. Heterosis as a consequence of regulatory incompatibility[J]. BMC Biology, 15: 38 [11] Hoecker N, Keller B, Muthreich N, et al.2008. Comparison of maize (Zea mays L.) F1-hybrid and parental inbred line primary root transcriptomes suggests organ-specific patterns of nonadditive gene expression and conserved expression trends[J]. Genetics, 179(3): 1275-1283. [12] Hoecker N, Keller B, Piepho H, et al.2006. Manifestation of heterosis during early maize (Zea mays L.) root development[J]. Theoretical and Applied Genetics, 112(3): 421-429. [13] Hollick J B, Chandler V L.1998. Epigenetic allelic states of a maize transcriptional regulatory locus exhibit overdominant gene action[J]. Genetics, 150(2): 891-897 [14] Jia L, Zhang B, Mao C, et al.2008. OsCYT-INV1 for alkaline/neutral invertase is involved in root cell development and reproductivity in rice (Oryza sativa L.)[J]. Planta, 228(1): 51-59. [15] Jones D F.1917. Dominance of linked factors as a means of accounting for heterosis[J]. Proceedings of the National Academy of Sciences of the USA, 3(4): 310-312 [16] Kurusu T, Nishikawa D, Yamazaki Y, et al.2012. Plasma membrane protein OsMCA1 is involved in regulation of hypo-osmotic shoch-induced Ca2+ influx and modulates generation of reactive oxygen species in cultured rice cells[J]. BMC Plant Biology, 12: 11. [17] Li D, Huang Z, Song S, et al.2016. Integrated analysis of phenome, genome, and transcriptome of hybrid rice uncovered multiple heterosis-related loci for yield increase[J]. Proceedings of the National Academy of Sciences of the USA, 113(41): E6026-E6035. [18] Liu Z, Cheng Q, Sun Y, et al.2015. A SNP in OsMCA1 responding for a plant architecture defect by deactivation of bioactive GA in rice[J]. Plant Molecular Biology, 87(1-2): 17-30. [19] Lv Q, Zhong Y, Wang Y, et al.2014. SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2 in rice[J]. The Plant Cell, 26: 1586-1597. [20] Ma Q, Hedden P, Zhang Q.2011. Heterosis in rice seedlings: its relationship to gibberellin content and expression of gibberellin metabolism and signaling genes[J]. Plant Physiology, 156(4): 1905-1920. [21] Miller M, Song Q, Shi X, et al.2015. Natural variation in timing of stress-responsive gene expression predicts heterosis in intraspecific hybrids of Arabidopsis[J]. Nature Communications, 6(1): 7453 [22] Moore S, Lukens L.2011. An evaluation of Arabidopsis thaliana hybrid traits and their genetic control[J]. G3-Genes, Genomes, Genetics, 1(7): 571-579 [23] Motte H, Vanneste S,Beeckman T.2019. Molecular and environmental regulation of root development[J]. Annual Review of Plant Biology, 70: 465-488. [24] Paschold A, Jia Y, Marcon C, et al.2012. Complementation contributes to transcriptome complexity in maize (Zea mays L.) hybrids relative to their inbred parents[J]. Genome Research, 22(12): 2445-2454. [25] Paschold A, Larson N B, Marcon C, et al.2014. Nonsyntenic genes drive highly dynamic complementation of gene expression in maize hybrids[J]. The Plant Cell, 26(10): 3939-3948. [26] Qi Y, Wang S, Shen C, et al.2012. OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa)[J]. New Phytologist, 193: 109-120. [27] Schnable P S, Springer N M.2013. Progress toward understanding heterosis in crop plants[J]. Annual Review of Plant Biology, 64: 71-88. [28] Shao L, Xing F, Xu C, et al.2019. Patterns of genome-wide allele-specific expression in hybrid rice and the implications on the genetic basis of heterosis[J]. Proceedings of the National Academy of Sciences of the USA, 116(12): 5653-5658. [29] Shull G H.1908. The composition of a field of maize[J]. Journal of Heredity, 4: 296-301 [30] Syed N H, Chen Z J.2005. Molecular marker genotypes, heterozygosity and genetic interactions explain heterosis in Arabidopsis thaliana[J]. Heredity, 94(3): 295-304. [31] Wang D, Pei K, Fu Y, et al.2007. Genome-wide analysis of the auxin response factors (ARF) gene family in rice(Oryza Sative)[J]. Gene, 394(1-2): 12-24. [32] Wang Y, Cai Q, Xie H, et al.2018. Determination of heterotic groups and heterosis analysis of yield performance in indica rice[J]. Rice Science, 25(5): 261-269. [33] Wang Y, Zheng Y, Cai Q, et al.2016. Population structure and association analysis of yield and grain quality traits in hybrid rice primal parental lines[J]. Euphytica, 212: 261-273. [34] Wang Z, Ruan W, Shi J, et al.2014. Rice SPX1 and SPX2 inhibit phosphate starvation response through interacting with PHR2 in a phosphate-dependent manner[J]. Proceeding of the National Academy of Sciences, 111(41): 14953-14958. [35] Werner T, Nehnevajova E, Köllmer I, et al.2010. Root-specific reduction of cytokinin causes enhanced root growth, drought tolerance, and leaf mineral enrichment in Arabidopsis and tobacco[J]. Plant Cell, 22: 3905-3920. [36] Wu P, Wang Z.2011. Molecular mechanisms regulating Pi-signaling and Pi homeostasis under OsPHR2, a central Pi-signaling regulator, in rice[J]. Frotiers in Biology, 6: 242-245. [37] Yang M, Wang X, Ren D, et al.2017. Genomic architecture of biomass heterosis in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the USA, 114(30): 8101-8106. [38] Yao S G, Kodama R, Wang H, et al.2009. Analysis of the rice SHORT-ROOTS gene revealed functional diversification of plant neutral/alkaline invertase family[J]. Plant Science, 176(5): 627-634. [39] Yao S G, Taketa S, Ichii M.2002. A novel short-root gene that affects speciffically early root development in rice (Oryza sativa L.)[J]. Plant Science, 163(2): 207-215. [40] Yoshida S, Fomo D A, Cock J H, et al.1976. Laboratory manual for physiological studies of rice[M]. The International Rice Research Institute, Laguna, Philippines, pp: 61-66. [41] Zhong Y, Wang Y, Guo J, et al.2019. Rice SPX6 negatively regulates the phosphate starvation response through suppression of the transcription factor PHR2[J]. New phytologist, 219(1): 135-148. [42] Zhu D, Zhou G, Xu C, et al.2016. Genetic components of ceterosis for seedling traits in an elite rice hybrid analyzed using an immortalized F2 population[J]. Journal of Genetics and Genomics, 43(2): 87-97.
