|
|
|
| Autophagy Participates in Abscisic Acid-mediated Salt Tolerance in Wheat (Triticum aestivum) |
| LI Yong-Bo1,*, CUI De-Zhou1,*, HUANG Chen1, SUI Xin-Xia1, FAN Qing-Qi1,**, CHU Xiu-Sheng1,2,** |
1 Crop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, China;
2 School of Life Science, Shandong Normal University, Jinan 250014, China |
|
|
|
|
Abstract Autophagy is a self-defense mechanism under environmental stress in plants. It has been proved that autophagy is involved in salt tolerance of wheat (Triticum aestivum), but the role and regulation mechanism of autophagy in salt tolerance are not clear. In this study, salt-tolerant wheat 'DEKANG961' and salt-intolerant wheat 'LANKAO323' were used as materials, and the methods of lysosomes' fluorescence probe, Western blot and so on were used to detect autophagy level. It was found that salt stress could promote autophagy, but the autophagy level of 'DEKANG961' was higher than that of 'LANKAO323', the gene expression of PYL1 (pyrabactin), PYL2, PYL3 and sucrose non-fermenting related protein kinase 2 (SNRK2), the key genes of abscisic acid (ABA) signaling pathway in 'DEKANG961', were significantly higher than that in 'LANKAO323', and the autophagy level of 'DEKANG961' was significantly higher than that of 'LANKAO323'. It was concluded that ABA signal pathway could promote the autophagy to enhance salt tolerance in wheat. Therefore, this study has certain theoretical and practical significance for further study on salt-tolerant mechanism of wheat and breeding new salt-tolerant wheat varieties.
|
|
Received: 11 May 2020
|
|
|
|
Corresponding Authors:
**xschu2007@sina.com; fanqingqi@163.com
|
|
|
|
[1] 丁同楼, 贾玉辉, 王宝山等. 2013. 不同耐盐性小麦根Na+和K+的吸收特性[J]. 植物生理学报, 49(1): 34-40.
(Ding T L, Jia Y H, WANG B S,et al.2013. Characteristics of Na+ and K+ uptake in roots of different salt-tolerant wheat cultivars[J]. Plant Physiology Journal, 49(1): 34-40.)
[2] 王芳, 段迪, 王宝山等. 2007. 不同耐盐性小麦胚芽鞘伸长对NaCl胁迫的响应[J]. 作物学报, 33(12): 2053-2058.
(Wang F, Duan D, Wang B S, et al.2007. Coleoptile elongation response of different salt-tolerant wheat cultivars to NaCl stress[J]. Acta Agronomica Sinica, 33(12): 2053-2058.)
[3] Clarke P G.1990. Developmental cell death: Morphological diversity and multiple mechanisms[J]. Anatomy and Embryology, 181(3): 195-213.
[4] Furihata T, Maruyama K, Yamaguchi-Shinozaki K, et al.2006. Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1[J]. Proceedings of the National Academy of Sciences of the USA, 103(6): 1988-1993.
[5] Genc Y, Oldach K, McDonald G K, et al.2010. Sodium exclusion QTL associated with improved seedling growth in bread wheat under salinity stress[J]. Theoretical and Applied Genetics, 121(5): 877-894.
[6] Huang L, Yu L J, Zhang X, et al.2019. Autophagy regulates glucose-mediated root meristem activity by modulating ROS production in Arabidopsis[J]. Autophagy, 15(3): 407-422.
[7] Hirayama T, Shinozaki K.2010. Research on plant abiotic stress responses in the post-genome era: Past, present and future[J]. The Plant Journal: for Cell and Molecular Biology, 61(6): 1041-1052.
[8] Huo L, Guo Z, Ma F, et al.2020. Increased autophagic activity in roots caused by overexpression of the autophagy-related gene MdATG10 in apple enhances salt tolerance[J]. Plant Science, 294: 110444.
[9] Inoue Y, Suzuki T, Moriyasu Y, et al.2006. AtATG genes, homologs of yeast autophagy genes, are involved in constitutive autophagy in Arabidopsis root tip cells[J]. Plant & Cell Physiology, 47(12): 1641-1652.
[10] Kumar M, Kesawat M S, Kim A H U, et al.2019. Integration of abscisic acid signaling with other signaling pathways in plant stress responses and development[J]. Plants, 8(12): 592-611.
[11] Liao C Y, Bassham D C.2020. Combating stress: The interplay between hormone signaling and autophagy in plants[J]. Journal of Experimental Botany, 71(5): 1723-1733.
[12] Liu Y, Bassham D C.2010. TOR is a negative regulator of autophagy in Arabidopsis thaliana[J]. PLOS ONE, 5(7): 11883-11891.
[13] Liu Y, Schiff M, Dinesh-Kumar S P, et al.2005. Autophagy regulates programmed cell death during the plant innate immune response[J]. Cell, 121(4): 567-577.
[14] Luo L, Zhang P, Gong Q.et al.2017. Autophagy is rapidly induced by salt stress and is required for salt tolerance in Arabidopsis[J]. Frontiers In Plant Science, 8: 1459.
[15] Martinez-Atienza J, Jiang X, Quintero F J, et al.2007. Conservation of the salt overly sensitive pathway in rice[J]. Plant Physiology, 143(2): 1001-1012.
[16] Miransari M, Smith D.2019. Sustainable wheat (Triticum aestivum L.) production in saline fields: A review[J]. Critical Reviews in Biotechnology, 39(8): 999-1014.
[17] Mostofa M G, Hossain M A, Fujita M.2015. Trehalose pretreatment induces salt tolerance in rice (Oryza sativa L.) seedlings: Oxidative damage and co-induction of antioxidant defense and glyoxalase systems[J]. Protoplasma, 252(2): 461-475.
[18] Niu M, Huang Y, Bie, Z, et al.2018. Root respiratory burst oxidase homologue-dependent H2O2 production confers salt tolerance on a grafted cucumber by controlling Na+ exclusion and stomatal closure[J]. Journal of Experimental Botany, 69(14): 3465-3476.
[19] Ohsumi Y.2014. Historical landmarks of autophagy research[J]. Cell Research, 24(1): 9-23.
[20] Osakabe Y, Yamaguchi-Shinozaki K, Tran L S, et al.2014. ABA control of plant macroelement membrane transport systems in response to water deficit and high salinity[J]. The New Phytologist, 202(1): 35-49.
[21] Pu Y, Luo X, Bassham D C.2017. TOR-dependent and -independent pathways regulate autophagy in Arabidopsis thaliana[J]. Frontiers in Plant Science, 8(7): 1204-1216.
[22] Shi H, Quintero F J, Zhu J K, et al.2002. The putative plasma membrane Na(+)/H(+) antiporter SOS1 controls long-distance Na(+) transport in plants[J]. The Plant Cell, 14(2): 465-477.
[23] Siddiqui M N, Mostofa M G, Akter M M, et al.2017. Impact of salt-induced toxicity on growth and yield-potential of local wheat cultivars: Oxidative stress and ion toxicity are among the major determinants of salt-tolerant capacity[J]. Chemosphere, 187(8): 385-394.
[24] Tang X, Mu X, Brestic M, et al.2015. Global plant-responding mechanisms to salt stress: Physiological and molecular levels and implications in biotechnology[J]. Critical Reviews in Biotechnology, 35(4): 425-437.
[25] Takatsuka C, Inoue Y, Moriyasu Y, et al.2004. 3-methyladenine inhibits autophagy in tobacco culture cells under sucrose starvation conditions[J]. Plant & Cell Physiology, 45(3): 265-274.
[26] Tanida I.2011. Autophagosome formation and molecular mechanism of autophagy[J]. Antioxidants & Redox Signaling, 14(11): 2201-2214.
[27] Toyooka K, Moriyasu Y, Matsuoka K, et al.2006. Protein aggregates are transported to vacuoles by a macroautophagic mechanism in nutrient-starved plant cells[J]. Autophagy, 2(2): 96-106.
[28] Urano K, Kurihara Y, Shinozaki K, et al.2010. 'Omics' analyses of regulatory networks in plant abiotic stress responses[J]. Current Opinion in Plant Biology, 13(2): 132-138.
[29] Vahisalu T, Kollist H, Kangasjarvi J, et al.2008. SLAC1 is required for plant guard cell S-type anion channel function in stomatal signaling[J]. Nature, 452(7186): 487-491.
[30] Wang P, Mugume Y, Bassham D C.2018. New advances in autophagy in plants: Regulation, selectivity and function[J]. Seminars In Cell & Developmental Biology, 80(8): 113-122.
[31] Xu X, Zhi T, Ji J, et al.2018. Corrigendum to ERK1/2/mTOR/Stat3 pathway-mediated autophagy alleviates traumatic brain injury-induced acute lung injury[J]. Biochimica et Biophysica Acta, 1864(5): 1663-1674.
[32] Yamauchi S, Mano S, Takemiya A, et al.2019. Autophagy controls reactive oxygen species homeostasis in guard cells that is essential for stomatal opening[J]. Proceedings of the National Academy of Sciences of the USA, 116(38): 19187-19192.
[33] Yang Y, Guo Y.2018. Elucidating the molecular mechanisms mediating plant salt-stress responses[J]. The New Phytologist, 217(2): 523-539.
[34] Yue W, Nie X, Song W.2018. Genome-wide sequence and expressional analysis of autophagy gene family in bread wheat (Triticum aestivum L.)[J]. Journal of Plant Physiology, 229(6): 7-21.
[35] Zeng X, Zeng Z, Han N, et al.2017. A barley homolog of yeast ATG6 is involved in multiple abiotic stress responses and stress resistance regulation[J]. Plant Physiology and Biochemistry, 115(3): 97-106.
[36] Zhu J K.2000. Genetic analysis of plant salt tolerance using Arabidopsis[J]. Plant Physiology, 124(3): 941-948.
[37] Zhu, J K.2003 Regulation of ion homeostasis under salt stress[J]. Current Opinion in Plant Biology, 6(5): 441-445. |
|
|
|
