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| Genetic Diversity Analysis of Fejervarya multistriata Population in Fanjingshan Region Based on 12S rRNA Sequence |
| WU Zhen-Yang1, LI Li1, ZHANG Lei1, QIU Fa-Gen1, YANG Shi-Long1, E Guang-Xin2,* |
1 Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren University, Tongren 554300, China;
2 College of Animal Science and Technology, Southwest University, Chongqin 400715, China |
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Abstract Fanjing mountain is the main peak of Wuling mountain range. It has rich amphibian diversity due to its diverse climate types and superior hydrothermal conditions. However, the related research mainly concentrated in the 1980's, and mostly based on field investigation. Therefore, it is urgent to evaluate the genetic diversity of amphibians in Fanjingshan region by using mitochondrial genes as analytical markers. To evaluate the genetic diversity of Fejervarya multistriata in the surrounding area of Fanjing mountain, the influence of the specific geographical environment of Fanjing mountain on its survival status, and to provide scientific basis for further protection and utilization of genetic resources of F. multistriata, the present study used partial sequences of mitochondrial DNA (mtDNA) 12S rRNA as molecular markers, 185 samples from 10 districts and counties in Fanjingshan area were sequenced and the genetic diversity was analyzed. The results showed that nucleotide diversity index Pi and haplotype diversity (Hd) were 0.029 42±0.000 34 and 0.563±0.017, respectively. Ten haplotypes were found, and the dominant haplotypes were haplotype 1 and 2, followed by haplotype 8, accounting for 36.76%, 44.32% and 12.43% of the total sample, respectively. Haplotype 1 and haplotype 8 were mainly composed of the populations east of Fanjing mountain, while haplotype 2 mainly existed in the population west of Fanjing mountain. Haplotype network mediation diagram showed that dominant haplotype 1 and haplotype 8 gathered into one class, and haplotype 2 gathered into another class. Hoplobatrachus chinensis and Nanorana ventripunctata were used as extranet to construct a haplotype systemic-occurrence tree, and the haplotype clustering analysis indicated that the haplotype cluster of Hoplobatrachus chinensis and F. multistriata were together, and the haplotype cluster of F. multistriata was divided into 2 subbranches, which was consistent with the results of haplotype network map and population phylogenetic tree, that was, the 10 populations of the F. multistriata could be divided into 2 branches as east and west of Fanjing mountain, indicating that it might be caused by the geographical isolation of Fanjing mountain vein. The fixed coefficient Fst indicated that the population differentiation degree was obvious in F. multistriata population. Analysis of molecular variance (AMOVA) showed that the genetic variation was mainly between populations (86.14%), while the genetic variation within populations was low (13.86%). Comprehensive analysis showed that the genetic diversity among different geographic populations of F. multistriata was relatively low, and genetic differentiation was obvious, which might be mainly due to the geographical isolation of complex landforms and mountains. The above results have some reference value for further research on the influence of complex geographical environment and climatic characteristics of Fanjing moutain area (such as the obvious vertical climate zone spectrum) on other amphibians.
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Received: 19 May 2020
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Corresponding Authors:
*eguangxin@126.com
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