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| Polymorphism of Gene Coding Region of BMPR-IB Gene and Its Correlation with Litter Size in Four Sheep (Ovis aries) Varieties |
| SUN Wei-Bo1,2, LIU Li-Shan1, ZHANG Li-Ping2, WANG Cai-Lian1, LANG Xia1,* |
1 Institute of Animal & Pasture Science and Green Agriculture, Gansu Academy of Agricultural Science / Key Laboratory for Sheep, Goat, and Cattle Germplasm and Straw Feed in Gansu Province, Lanzhou 730070, China;
2 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China |
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Abstract In order to explore the genetic effect of polymorphism in the bone morphogenetic protein receptor-IB (BMPR-IB) gene coding region on the reproductive performance of sheep (Ovis aries), ewes of 4 sheep breeds were used as the research object in this study, which includes 65 multiple Small Tail Han sheep (SM), 71 twins Mongolian sheep (MT), 70 singleton Mongolian sheep (MS), 52 twins Oula sheep (ZT), 51 singletons Oula sheep (ZS), and 74 singletons Dorper sheep (DS). Using PCR combined DNA direct sequencing method, the polymorphism of 597 loci in the coding region of BMPR-IB gene of different fertility ewes was tested, and the correlation analysis was performed with the litter size. The results showed that: G/A mutation was detected at the 597 site of the coding region of the BMPR-IB gene, GG, GA and AA genotypes were detected in each experimental group sheep. The dominant genotype in SM was AA, and the dominant allele was A. The dominant genotype in MT, MS, ZT and ZS was GA, the dominant allele was G. The dominant genotype in DS was GG, and the dominant allele was G. All the experimental group sheep were in moderate polymorphism (0.25<PIC<0.5); the litter size of homozygous mutation AA type was significantly higher than that wild GG type and heterozygous mutant GA type in the Oula sheep experimental population (P<0.05). Therefore, this locus can be used as a potential molecular genetic marker site for sheep lambing number. This study provides theoretical support for the genetic mechanism and breeding of sheep lambing traits.
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Received: 08 June 2020
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Corresponding Authors:
*langxiax@163.com
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[1] 陈晓勇, 敦伟涛, 田树军, 等. 2012. FecB基因对绵羊生产性能影响的研究进展[J]. 中国草食动物科学, 32(04): 59-65.
(Chen X Y, Guo W T, Tian S J, et al.2012. Review on the effect of FecB gene on the production performance of sheep[J]. China Herbivore Science, 32(04): 59-65.)
[2] 董明, 李会成. 2004. 转化生长因子β的研究进展[J]. 诊断学理论与实践, (01): 49-52.
(Dong M, Li H C. 2004. Research progress of transforming growth factor β[J]. Journal of Diagnostics Concepts & Practice, (01):49-52.)
[3] 杜丽. 2018. 绵羊BMPR-IB和GDF9基因SNPs及GDF9基因mRNA表达量分析[D]. 硕士学位论文, 甘肃农业大学, 导师: 张利平, pp. 39-41.
(Du L.2018. SNPs analysis of BMPR-IB and GDF9 genes in sheep and analysis of GDF9 mRNA expression level[D]. Thesis for M.S., Gansu Agricultural University, Suppervisor: Zhang L P, pp. 39-41.)
[4] 管峰. 2005. 绵羊多产性状的DNA分子标记及相关研究[D]. 博士学位论文, 南京农业大学, 导师: 杨利国, pp.38-40.
(Guan F.2005. Study on DNA molecular markers and its related research on prolificacy in sheep[D], Thesis for Ph.D., Nanjing Agricultural University, Suppervisor: Yang L G, pp.38-40.)
[5] 焦丹, 刘秀, 李少斌, 等. 2016. 绵羊BMPR1B基因的生物信息学分析[J]. 甘肃农业大学学报, 51(04): 1-6.
(Jiao D, Liu X, Li S B, et al.2016. Bioinformatics analysis of BMPR1B gene in sheep[J]. Journal of Gansu Agricultural University, 51(04):1-6.)
[6] 李桂英, 张军. 2014. 影响高寒牧区细毛羊繁殖力的主要因素及应对措施[J]. 养殖与饲料, (07):25-27.
(Li G Y, Zhang J. 2014. Main factors affecting reproductive capacity of Fine wool sheep in alpine pasture areas and countermeasures[J]. Animals Breeding and Feed, (07):25-27.)
[7] 林海燕, 王红梅, 祝诚. 2003. 转化生长因子-β信号传导的Smad通路[J]. 中国科学(C辑: 生命科学), (02): 97-109.
(Lin H Y, Wang H M, Zhu C. 2003. Smad pathway of transforming growth factor-β signaling[J]. Science in China Series C-Life Sciences, (02):97-109.)
[8] 柳淑芳, 姜运良, 杜立新. 2003. BMPR-IB和BMP15基因作为小尾寒羊多胎性能候选基因的研究[J]. 遗传学报, (08): 755-760.
(Liu S F, Jiang Y L, Du L X. 2003. Studies of BMPR-IB and BMP15 as candidate genes for fecundity in little tailed han sheep[J], Acta Genetica Sinica, (08):755-760.)
[9] 柳树群, 刘次全. 1999. mRNA的序列,结构以及翻译速率与蛋白质结构的关系[J]. 动物学研究, (6):457-461.
(Liu S Q, Liu C Q. 1999. The relationship between mRNA's sequence, structure, translational rate and protein structure[J]. Zoological Research, (6):457-461.)
[10] 马吉锋, 王建东, 张俊丽, 等. 2016. 滩湖后代和双胎滩公羊多羔主效基因FecB检测试验[J]. 中国畜禽种业, 12(10): 57-58.
(Ma J F, Wang J D, Zhang J L, et al.2016. Detection experiment of FecB gene of multi-lamb of Tanhu offspring and twins rams[J]. The Chinese Livestock and Poultry Breeding, 12(10): 57-58.)
[11] 潘章源, 狄冉, 刘秋月, 等. 2015. 绵羊多羔主效基因BMPR1B的研究进展[J]. 家畜生态学报, 36(05):1-6.
(Pan Z Y, Di R, Liu Q Y, et al.2015. Research progress of sheep multi-lamb major gene BMPR1B[J]. Journal of Domestic Animal Ecology, 36(05): 1-6.)
[12] 邱寒峰, 徐雅萍, 吴婷, 等. 2019. 骨形态发生蛋白15和生长分化因子9对家畜繁殖功能的影响[J].浙江畜牧兽医, 44(05): 9-11.
(Qiu H F, Xu Y P, Wu T, et al.2019. Effects of bone morphogenetic protein 15 and growth differentiation factor 9 on livestock reproductive function[J]. Zhejiang Journal Animal Science and Veterinary Medicine, 44(05): 9-11.)
[13] 史洪才, 武坚, 朱二勇, 等. 2006. BMPR-IB基因作为新疆多浪羊多胎性能候选基因的研究[J]. 中国草食动物, (02): 12-14.
(Shi H C, Wu J, Zhu E Y, et al. 2006. Study on BMPR-IB gene as a candidate gene for multiple fertility in xinjiang duolang sheep[J]. China Herbivores, (02): 12-14.)
[14] 王根林, 毛鑫智, Davis G H, 等. 2003. DNA分析发现我国湖羊和小尾寒羊存在Booroola (FecB)多胎基因[J]. 南京农业大学学报, (01): 104-106.
(Wang G L, Mao X Z, Davis G H, et al.2003. DNA analysis found Booroola (FecB) multi-fetal genes in Hu sheep and small-tailed Han sheep in China[J]. Journal of Nanjing Agricultural University, (01): 104-106.)
[15] 王启贵, 钟发刚, 李辉, 等. 2003. 绵羊BMPR-IB基因多态性与其产羔数的相关研究[J]. 草食家畜, (02): 20-23.
(Wang Q G, Zhong F G, Li H, et al. 2003. The polymorphism of BMPR-IB gene associated with litter size in sheep[J]. Grass-feeding livestock, (02): 20-23.)
[16] 闫亚东, 储明星, 曾勇庆, 等. 2005. 小尾寒羊和湖羊高繁殖力候选基因BMPR-IB的研究[J]. 农业生物技术学报, (01): 66-71.
(Yang Y D, Chu M X, Zeng Y Q, et al. Study on Bone Morphogenetic Protein Receptor IB as a candidate gene for prolificacy in Small tail han sheep and Hu sheep[J]. Journal of Agricultural Biotechnology, (01): 66-71.)
[17] 杨燕燕, 田瑛, 达来, 等. 2010. BMPR-IB基因作为影响蒙古羊双羔性状候选基因的研究[J]. 黑龙江畜牧兽医, (11): 1-4.
(Yang Y Y, Tian Y, Da L, et al. 2010. Study on the BMPR-IB gene as candidate gene for fecundity in Mongolian sheep double-lambs group[J]. Heilongjiang Animal Science and Veterinary Medicine, (11): 1-4.)
[18] 杨媛, 翁恺麒, 李拥军, 等. 2017. BMPR-IB基因多态性与湖羊产羔性能的关系[J]. 安徽农业科学, 45(26): 113-115.
(Yang Y, Weng K Q, Li Y J, et al.2017. Relationship between polymorphism of BMPR-IB gene and lambing performance of Hu sheep[J]. Journal of Anhui Agricultural Sciences, 45(26): 113-115.)
[19] 张舒. 2007. 遗传密码子的研究历史及进展概述[J]. 科技创业月刊, (11): 182-183.
(Zhang S. 2007. Overview of the research history and progress of genetic code[J]. Ploneering with Science & Technology Monthly, (11): 182-183.)
[20] 朱韶华, 张利平, 马晓明, 等. 2018. 绵羊BMP-15基因在不同产羔性状母羊中的表达量差异及分析[J]. 基因组学与应用生物学, 37(05): 1853-1858.
(Zhu S H, Zhang L P, Ma X M, et al.2018. Analysis and differences of sheep BMP-15 gene expression in ewes with different lambing traits[J]. Genomics and Applied Biology, 37(05): 1853-1858.)
[21] Chu M X, Jia L H, Zhang Y J, et al.2011. Polymorphisms of coding region of BMPR-IB gene and their relationship with litter size in sheep[J]. Molecular Biology Reports, 38(6): 4071-4076.
[22] Davis G H, Balakrishnan L, Ross I K, et al.2006. Investigation of the Booroola (FecB) and Inverdale (FecX(I)) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries[J]. Animal Reproduction Science, 92(1-2): 87-96.
[23] Fogarty N M.2009. A review of the effects of the Booroola gene (FecB) on sheep production[J]. Small Ruminant Research, 85(2-3): 75-84.
[24] Guan F, Liu S R, Shi G Q, Yang L G.2007. Polymorphism of FecB gene in nine sheep breeds or strains and its effects on litter size, lamb growth and development[J]. Animal Reproduction Science, 99(1-2): 44-52.
[25] Iwasaki S, Hattori A, Sato M, et al.1996. Characterization of the bone morphogenetic protein-2 as a neurotrophic factor. Induction of neuronal differentiation of PC12 cells in the absence of mitogen-activated protein kinase activation[J]. Journal of Biological Chemistry, 271(29): 17360-17365.
[26] Kimchi-Sarfaty C, Oh J M, Kim I W, et al.2007. A "Silent" polymorphism in the MDR1 gene changes substrate specificity[J]. Animal Science, 315(5811): 525-528.
[27] McNatty K P, Juengel J L, Wilson T, et al.2001. Genetic mutations influencing ovulation rate in sheep[J]. Reproduction, fertility, and development, 13(7-8): 549-555.
[28] Mehler M F, Mabie P C, Zhang D, et al.1997. Bone morphogenetic proteins in the nervous system[J]. Trends in neurosciences, 20(7): 309-317.
[29] Mishina Y, Suzuki A, Ueno N, et al.1995. Bmpr encodes a type I bone morphogenetic protein receptor that is essential for gastrulation during mouse embryogenesis[J]. Genes & development, 9(24): 3027-3037.
[30] Mulsant P, Lecerf F, Fabre S, et al.2001. Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Mérino ewes[J]. Proceedings of the National Academy of Sciences of the USA, 98(9): 5104-5109.
[31] Shimasaki S, Zachow R J, Li D, et al.1999. A functional bone morphogenetic protein system in the ovary[J]. Proceedings of the National Academy of Sciences of the USA, 96(13): 7282-7287.
[32] Souza C J, MacDougall C, MacDougall C, et al.2001. The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B (BMPR1B) gene[J]. Journal of Endocrinology, 169(2): R1-R6.
[33] Telling G C, Parchi P, DeArmond S J, et al.1996. Evidence for the conformation of the pathologic isoform of the prion protein enciphering and propagating prion diversity[J]. Science, 274(5295): 2079-2082.
[34] Wang W M, Liu S J, Li F D, et al.2015. Polymorphisms of the Ovine BMPR-IB, BMP-15 and FSHR and their associations with litter size in two Chinese indigenous sheep breeds[J]. International Journal of Molecular Sciences, 16(5): 11385-11397.
[35] Yamada H, Obata H, Kaji Y, et al.1999. Expression of transforming growth factor-β superfamily receptors in developing rat eyes[J]. Japanese Journal of Ophthalmology, 43(4): 290-294.
[36] Yi S E, LaPolt P S, Yoon B S, et al.2001. The type I BMP receptor BMPR-IB is essential for female reproductive function[J]. Proceedings of the National Academy of Sciences of the USA, 98(14): 7994-7999. |
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