割手密CDPK基因家族的鉴定及表达分析

doi:10.3969/j.issn.1674-7968.2020.12.006

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Abstract Calcium-dependent protein kinases (CDPKs or CPKs) function as Ca²⁺ sensors and effectors to directly translate Ca²⁺ signals into downstream phosphorylation signals. CDPKs are involved in plant growth and developmental processes, as well as responses to abiotic and biotic stresses. However, little information is available about the CDPK family in Saccharum plants. In this study, based on the published Saccharum spontaneum genome data, the CDPK gene family members of S. spontaneum were identified by bioinformatics methods, and a total of 90 CDPKs were identified, whose physical and chemical parameters, chromosomal locations, gene duplication, phylogenetic relationships, evolutionary characteristics, gene structure, and motif distribution were analyzed. Syntenic analysis showed that consensuses in CDPKs might have existed before the species divergence between S. spontaneum and Oryza sativa . The selection pressure analysis showed that CDPK genes in S. spontaneum were positively selected in the process of evolution and might play important roles in the evolution of plants. Transcriptome analysis showed that most SsCDPKs exhibited different expression levels in different tissues and developmental stages. In addition, many SsCDPKs in sugarcane hybrid variety Yuetang 55 (YT55) exhibited different expression profile under low-potassium and low-nitrogen treatments. The diverse expression of SsCDPKs suggested potential important function of SsCDPKs in regulating the development and responses to low-potassium and low-nitrogen stresses of Saccharum plants. This study provides a reference for further analysis of roles of Saccharum CDPK genes in the development and responses to nutrition stresses.

Key words： Saccharum Calcium-dependent protein kinase (CDPK) Bioinformatics analysis Growth and development Nutrient stress

Received: 28 May 2020

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S184

Corresponding Authors: *yongwen2001@126.com

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	Articles by authors
	ZHANG Nan-Nan
	HUANG Yong-Hong
	QI Peng
	FENG Xiao-Min
	WU Zi-Lin
	ZENG Qiao-Ying
	QI Yong-Wen

Cite this article:

ZHANG Nan-Nan,HUANG Yong-Hong,QI Peng, et al. Identification and Expression Analysis of CDPK Gene Family in Saccharum spontaneum[J]. 农业生物技术学报, 2020, 28(12): 2151-2164.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.12.006 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I12/2151

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