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| Exploring PacC Mediated Pathogenic Mechanism of Valsa mali Based on Transcriptome |
| ZHU Shan*, WANG Yi-Bo*, LI Jian-Yu, XIAO Ying-Zhu, XU Liang-Sheng**, HUANG Li-Li** |
| College of Plant Protection, Northwest A & F University, State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China |
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Abstract Apple tree canker caused by Valsa mali seriously restricts the development of apple industry in China. It is of great theoretical significance for the effective prevention and control of apple tree canker to comprehensively analyze the pathogenic molecular mechanism of V. mali. The pH-responsive transcription factor PacC play an important role in the infection and pathogenic process of V. mali. In order to reveal PacC transcriptional regulation mechanism, the gene expression profiles of V. mali wild-type 03-8 and the PacC deletion mutant ΔVmPacC on branches were analyzed by RNA-seq assays. The results showed that there were 238 and 449 differentially expressed genes (DEGs) during pre-infection and post-infection of V. mali. GO enrichment analysis showed that biological processes of DEGs were mainly involved in carbohydrate metabolic processes, oxidation-reduction process, transmembrane transport process and polysaccharide metabolic processes. KEGG functional enrichment analysis showed that the pathways of DEGs were mainly involved in starch and sucrose metabolism, cyanoamino acid metabolism, pentose and glucuronate interconversions, these DEGs involved in three pathways were mainly related to plant cell wall polysaccharide (PCWP) degradation. The amino acid sequences of DEGs involved PCWP degradation were submitted to Pathogen Host Interactions (PHI)-base blast, that obtained 3 potential pathogenic related genes including putative pectate lyase A gene, pectin lyase B gene, hypothetical protein gene VM1G_08430. In this study, six DEGs were selected for qRT-PCR verification, and the results showed that the mRNA expression patterns of the 6 genes were consistent with the results of RNA-seq assays. The comprehensive analysis showed that transcription factor PacC functions in the infection and pathogenic process by regulating the expression of PCWP degradation genes. The study revealed PacC transcriptional regulation mechanism in the infection and pathogenic process of V. mali, and it would lay a theoretical foundation a theoretical basis for explaining the pathogenic mechanism of V. mali.
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Received: 17 April 2020
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Corresponding Authors:
**liangsheng.xu@nwafu.edu.cn; lilyhuangk@163.com
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| About author:: * The authors who contribute equally |
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