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| Genome-wide Association Study of Morphometric Traits in Horse (Equus caballus) |
| LIU Ling-Ling, MENG Jun, WANG Qiong, CAO Hang, LV Shi-Peng, LIU Wu-Jun*, YAO Xin-Kui* |
| College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China |
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Abstract Morphometric influences sports performance,new techniques of genome-wide association study (GWAS) for morphometric trait of horse introduce new possibilities for sports performance. The aim of this study was to enrich the contents of morphometric traits and sport performance of horses (Equus caballus),it can explore the regulation genes of sport performance. In this study,GWAS methods were used to seek genetic markers and functional genes that affect the morphology traits of horses. A GWAS for morphometric traits (withers height (WH),body length (BL),cannon circumference (CC),croup height (CH),body length ratio (BLR),heart girth ratio (HGR),cannon circumference ratio (CCR) were conducted in 4 breeds of 157 individual horses (Yili horses,Thoroughbred horse,Russian speed horse and Orlov horse) which were genotyped using the Affymetrix Equine SNP 670 chip. Plink1.9 software was used to quality control for SNPs,after quality control,494 137 SNPs were participated in association analysis. Databases of Ensembl were used to carry out gene annotation and gene function analyses. Through Bonferroni correction at the genomic level,3 SNPs,15 SNPs,1 SNPs,1 SNPs and 9 SNPs were potentially significantly associated with the 5 traits of WH,CC,CH,HGR,CCR,respectively (P<9.29E-06). However,no SNPs were associated with BL and BLR. It was found that the 2 genes UBE2E3 and LUM regulated the sport performance of horses,which provided a reference for the application of marker-assisted selection in horse breeding.
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Received: 06 January 2020
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Corresponding Authors:
*lwj_ws@163.com;yxk61@126.com
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