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| Proteomics Study on Meat Quality of Myostatin (MSTN) Gene-edited Cattle (Bos taurus) |
| LI Xin, HAI Chao, LIU Chun-Li, BAI Chun-Ling, WEI Zhu-Ying, LI Guang-Peng, ZHAO Yue-Fang* |
| State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock,Inner Mongolia University,Hohhot 010070,China |
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Abstract The myostatin gene (MSTN) is a negative regulator of skeletal muscle development,and its function lose in mammals leads to overdevelopment of muscle,significant improvement of production performance,and certain effects on meat quality traits. The present study utilized the muscle tissues from MSTN gene edited Luxi beef cattle (Bos taurus) (Exp for short) and wild type (Ctr for short) to perform label free quantitative comparative proteomics,and investigate the effect of MSTN artificial mutation on meat quality traits. A total of 1 595 quantified proteins containing at least one unique peptide per protein were identified. After scatter plotting analysis used for determining the internal error of the biological replicates and student t-test analysis of the data set,the proteins with fold change of Exp/Ctr in relative abundance more than 1.5 or less than 0.67 and a P-value less than 0.05 from t-test were identified as differentially expressed proteins (DEPs). As a result,160 DEPs were identified which contained 28 proteins in increased abundance and 132 in decreased abundance. Gene Ontology (GO) enrichment and KEGG pathway analysis were performed to explore molecular mechanism of DEPs in the determination of meat quality. There were 5 GO terms of biological process,7 of cellular component,and 5 of molecular function enriched significantly. The biological process were related to oxidation reduction process,skeletal muscle contraction,transition between fast and slow fiber,mitochondrial electron transport nicotinamide adenine dinucleotide (NADH) to ubiquinone,cardiac muscle hypertrophy in response to stress. The cellular component were extracellular exosome,mitochondrial respiratory chain complexⅠ,mitochondrion,Z disc,focal adhesion,extracellular matrix,mitochondrial inner membrane. The molecular function were actin binding,NADH dehydrogenase (ubiquinone) activity,protein C-terminus binding,structural constituent of muscle,thioredoxin peroxidase activity. KEGG pathway analysis results included non-alcoholic fatty liver disease (NAFLD),oxidative phosphorylation,Alzheimer's disease,Parkinson's disease,Huntington's disease. Among the DEPs there were 27 proteins related to meat quality traits,which had been reported. The proteins related with muscle fiber were MYH (myosin heavy chain) 1,MYH6,and MYL6 (myosin light polypeptide 6),MYBPH (myosin binding protein H) and MYOM2 (myomesin (M-protein) 2). The proteins related to adipogenesis were ADIPOQ (adiponectin),CDH13 (cadherin-13),ECH1 (enoyl coenzyme A hydratase 1),CAT (catalase) and VIM (vimentin),which were all down-regulated. Nine proteins of mitochondrial NADH dehydrogenase were also down-regulated,containing NDUFC2/B2/S6/V3/A13/B5/S3/B9/S8. To illustrate the regulation information,interaction networks of DEPs and MSTN were analyzed through the STRING database. The results revealed that the 27 DEPs related to meat quality traits all interacted with MSTN,of which MYH6,MYH1 and ADIPOQ interacted with MSTN directly. By combination of the results reported in this work and previous studies,it could be concluded that MSTN might play a critical role in muscle development and energy metabolism and affect the beef quality traits by changing the types and composition of muscle fibers,reducing intramuscular fat synthesis and decreasing oxidative characteristics. The present research would facilitate further studies on the biological response of postmortem meat and provide further insight into the molecular mechanisms of proteins in the regulation of meat quality traits.
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Received: 27 April 2020
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Corresponding Authors:
*nklana@163.com
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