Cloning of 3 PIPs from Ricinus communis and Expression Analysis Under Cold Stresses
LIU Xu-Ming1,2, LI Min3, ZHANG Man4, HUO Hong-Yan1, HE Zhi-Biao5, ZHANG Ji-Xing1,2,6,*, WANG Xiao-Yu1,2,6,*
1 College of Life Science and Food, Inner Mongolia University for Nationalities, Tongliao 028000, China; 2 Horqin Plant Stress Biology Research Institute, Inner Mongolia University for Nationalities, Tongliao 028000, China; 3 Agricultural college, Inner Mongolia University for Nationalities, Tongliao 028000, China; 4 Baicheng Institute of Agricultural Sciences, Baicheng 137000, China; 5 Tongliao Institute of Agricultural Sciences, Tongliao 028000, China; 6 Inner Mongolia Industrial Engineering Research Center of Universities for Castor/Inner Mongolia Key Laboratory of Castor Breeding/Inner Mongolia Collaborative Innovation Cultivate Center for Castor, Tongliao 028000, China
Abstract Plant aquaporin is involved in rapid transmembrane transport of water and regulates various physiological processes such as nutrient transport, seed germination and lateral root growth in plants. In this study, three plasma membrane intrinsic proteins, RcPIP1;3 (Ricinus communis plasma membrane intrinsic proteins genes 1; 3)(GenBank No. MT068547), RcPIP2;1 (GenBank No. MT068546) and RcPIP2;2 (GenBank No. MT068545), were isolated from leaves of Tongbi 5 genotype by RT-PCR. Bioinformatic analysis showed that these 3 genes all contained 4 exons and 3 introns, 6 transmembrane helics and 2 specific NPA (Asn-Pro-Ala) motifs of major intrinsic proteins (MIPs) superfamily. Multiple sequence alignment indicated the homology of RcPIP1;3 with PtPIP1;3 reached 92.36%, and the homology of RcPIP2;1 and RcPIP2;2 with PtPIP2;1 and PtPIP2;2 were as high as 87.80% and 89.58%. Phylogenetic analysis confirmed RcPIP1;3 was the member of PIP1s family, RcPIP2;1 and RcPIP2;2 were the members of the PIP2s family. Sequence analysis of the promoter region showed that the 3 genes had cis-acting elements in response to hormone induction and environmental stress, which would suggested that they might have potential functions of regulating growth and development and adapting to environmental stress. qRT-PCR analysis found that RcPIP1;3 and RcPIP2;2 were up-regulated by cold stress, and RcPIPP2;1 was down-regulated by cold stress, which indicated that 3 genes may have antagonistic regulation modes in response to cold stress. This research provides an important theoretical basis for exploring the cold adaptation regulation process mediated by RcPIPs.
LIU Xu-Ming,LI Min,ZHANG Man, et al. Cloning of 3 PIPs from Ricinus communis and Expression Analysis Under Cold Stresses[J]. 农业生物技术学报, 2020, 28(10): 1788-1797.
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