香鱼白细胞介素-1受体相关激酶3 (IRAK-3)的序列特征及对鳗弧菌感染的免疫相关性分析

doi:10.3969/j.issn.1674-7968.2020.10.001

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Abstract Interleukin-1 receptor-associated kinase-3 (IRAK-3) is a member of the IRAK family. It can regulate immune homeostasis and tolerance in a number of infectious and non-infectious diseases through its inhibition of pro-inflammatory cytokine production. Recently, few studies are focusing on the function of IRAK-3 in fish. Herein, an IRAK-3 transcript (PaIRAK-3) was obtained from the monocyte/macrophage transcriptome of ayu (Plecoglossus altivelis). It possessed an open reading frame of 1 722 bp, predictedly encoding 573 amino acids, and had a theoretical molecular weight of 63.2 kD. PaIRAK-3 exhibited a N-terminal death domain, a proline/serine/threonine-rich domain, a kinase and/or pseudokinase domain and a C-terminal domain of IRAK family. The phylogenetic tree showed that IRAK-3 was relatively conservative in fish, and PaIRAK-3 had the highest homology with rainbow trout (Oncorhynchus mykiss) (63.1%) and silver salmon (Oncorhynchus kisutch) (63.0%). Quantitative PCR analysis showed that PaIRAK-3 was mainly expressed in the kidney, gill, spleen, liver and intestine. After Vibrio anguillarum infection, PaIRAK-3 was significantly up-regulated at the mRNA level in the kidney, spleen and liver of ayu, reaching the maximum value at 8 h post infection. And the mRNA level of PaIRAK-3 increased only at 8 h post infection in the gill tissue. Recombinant PaIRAK-3 (rPaIRAK-3) was prokaryotically expressed using BL21 Rosetta strain of Escherichia coli, and the purified rPaIRAK-3 was used to immunize mice (Mus musculus) to prepare the polyclonal antibodies. Western blot analysis using the polyclonal antibodies against rPaIRAK-3 showed that PaIRAK-3 was significantly up-regulated against V. anguillarum infection at the protein level in the liver and spleen tissue and reached the maximum value at 12 h post infection. In conclusion, PaIRAK-3 is closely related to V. anguillarum infection, which provides a premise for further research on the antibacterial immunity of PaIRAK-3.

Key words： Interleukin-1 receptor-associated kinase (IRAK-3) Ayu Vibrio anguillarum Immunity

Received: 20 February 2020

ZTFLH:

S917.4

Corresponding Authors: * zhouqianjin@nbu.edu.cn

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	LI Shang-Yang
	ZHOU Yan
	CHAI Fang-Chao
	ZHOU Qian-Jin
	CHEN Jiong

Cite this article:

LI Shang-Yang,ZHOU Yan,CHAI Fang-Chao, et al. Sequence Characteristics of Interleukin-1 Receptor-Related Kinase 3 (IRAK-3) in Ayu (Plecoglossus altivelis) and Its Immune Correlation Analysis Against Vibrio anguillarum Infection[J]. 农业生物技术学报, 2020, 28(10): 1711-1721.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.10.001 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I10/1711

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