Effect of NONHSAT184603.1 Gene on the Expression of Helicase Gene BLM
YANG Li-Hong1, 2, 3, WU Xiao-Min1, 2, 4, CUI Xiao-Zhi1, 2, 4, WU Qiao-Qun1, 2, 4, LUO Bin-Jie1, 2, 3, CHEN Ying-Lian1, 2, 5, XU Hou-Qiang1, 2, 4, *
1 Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Life Sciences, Guizhou University, Guiyang 550025, China; 2 Key Laboratory of Animal Genetics, Breeding and Reproduction in Guizhou Province,College of Life Sciences, Guizhou University, Guiyang 550025, China; 3 College of Life Science, Guizhou University, Guiyang 550025, China; 4 College of animal science, Guizhou University, Guiyang 550025, China; 5 College of Medical, Guizhou University, Guiyang 550025, China
Abstract BLM (bloom helicase) plays an important role in cell proliferation and growth. YAP (yes associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) porteins, as transcriptional coactivators and key components downstream of the mammalian Hippo signal pathway, are involved in the regulation of cell proliferation, apoptosis, migration and other physiological processes.And the long non-coding RNA (LncRNA) is very critical for regulating gene expression in cell growth and proliferation. To explore LncRNAs effect on gene expression of BLM, YAP, TAZ and screen BLM helicase related LncRNAs, fluorescence quantitative PCR technique had been used to detect the expression of selected LncRNA in 4 kinds of cell lines (22RV-1, PC3, LNCap, WPMY-1). Construction of LncRNA over-expression vector had been transfected in PC3 cells and WPMY-1 to detect the impact on BLM, YAP and TAZ gene after transfection LncRNA 24 and 48 h by using the fluorescent quantitative PCR technique. The NONHSAT184603.1 (abbreviated as N183) (GenBank No. m121218_085412_00126_c100) was successfully screened as candicate. The fluorescence quantitative PCR results showed that the expression of N183 in 22RV-1 and LNCap cells was significantly lower than that in normal prostate epithelial cell line WPMY-1 (P<0.01), and the expression in PC3 cells was significantly higher than that in WPMY-1 (P<0.01). After transfection with N183 over-expression vector, compared with the control group, the expression of BLM and TAZ were significantly down-regulated 24 h after transfection (P<0.01), and the expression of BLM and YAP were significantly up-regulated 48 h after transfection (P<0.01). BLM expression was significantly down-regulated (P<0.05), but there were no significant difference in YAP and TAZ expression in PC3 cells 48 h after transfection with N183 over-expression vector (P>0.05).The above results indicated that N183 could inhibit BLM gene expression in prostate cancer cells and normal cells but had different effect no YAP and TAZ genes, which made a contribution of basic data for anti-cancer researches by targeting BLM helicase.
YANG Li-Hong,WU Xiao-Min,CUI Xiao-Zhi, et al. Effect of NONHSAT184603.1 Gene on the Expression of Helicase Gene BLM[J]. 农业生物技术学报, 2020, 28(9): 1642-1651.
