Cloning and Expression Analysis of AcLOX2 Gene in Onion (Allium cepa)
GAO Lu-Yao1, 2, LI Qian1, 3, ZHOU Xue-Mei1, 2, WANG Yong2, HUANG Ke3, *, WANG Yong-Qin1, *
1 Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences / Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing 100097, China; 2 College of Horticulture, Northeast Agricultural University, Harbin 150030, China; 3 College of Horticulture,, Hunan Agricultural University, Changsha 410128, China;
Abstract Lipoxygenases (LOXs) are a class of non-heme and iron-containing fatty acid dioxygenases that play a key role in the lipid oxidation process and have an impact on the seed aging process. Onion (Allium cepa) seeds are short-lived, which have a serious impact on actual production research. In order to study the effect of LOX gene on the development and deterioration of onion seed, in this study, onion was used as the experimental material to clone the cDNA region of AcLOX (GenBank No. KX427168.1) by rapid amplification of cDNA ends (RACE) technology. Bioinformatics analysis was used to study its gene sequence characteristics; subcellular localization was used to study its expression site. qRT-PCR was used to identify the expression pattern of AcLOX in different parts of onion seeds at different stages. The results showed that AcLOX2 was 3 087 bp in length and contained a 2 727 bp ORF which encoding 908 amino acids. Subcellular localization revealed that AcLOX2 was located in mitochondria. qRT-PCR results indicated that AcLOX2 was expressed in all tissues measured, and its expression level was positive related to aging time. This experiment preliminarily confirmed that AcLOX2 played an important role in the aging process of onion seeds, and lays a foundation for studying the mechanism of short-lived mechanism of onion seeds.
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