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| Tissue Expression of LncRNA XLOC_15448 in Youzhou Dark Goat (Capra hircus) and Prediction of Its Interaction Molecules in Melanoma Pathogenesis |
| FU Lin, REN Hang-Xing, WANG Gao-Fu, JIANG Jing, LI Jie, SUN Xiao-Yan, LIU Liang-Jia, ZHOU Peng, ZHANG Li* |
| 1 Chongqing Academy of Animal Sciences/Chongqing Engineering Research Center for Goat, Rongchang 402460, China |
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Abstract Long noncoding RNA (LncRNA) was widely transcribed in the mammalian genome. LncRNA can play biological functions through the corresponding micRNA and target genes. By combining bioinformatics prediction and experimental verification, the LncRNA target genes can be rapidly and efficiently obtained and screened. To further explore the expression characteristics and predict its interaction molecules in melanoma pathogenesis of LncRNA XLOC_15448, the tissue expression profile LncRNA XLOC_15448 was detected using qRT-PCR method in different tissues of Youzhou dark goat (Capra hircus) and Youzhou blank goat (Capra hircus), as well as the LncRNA XLOC_15448 expression characteristics were detected in B16 cells at different developmental stages. The target genes were also predicted using miRBase, TargetScan7.2, miRTarBase, RNA22 version 2.0, and RNAhybrid2.2, and finally the lncRNA-miRNA-mRNA regulatory network was constructed in Capra hircus. The results demonstrated that LncRNA XLOC_15448 was widely expressed in different tissues of the goat, especially with the highest expression in the heart and lowest level in spleen, and it was also widely expressed in muscle, brain, skin, liver, kidney, lung and spleen. Comparison analysis illustrated that the skin LncRNA XLOC_15448 was expressed significantly higher in Youzhou dark goat than that of Youzhou blank white goat, and that the expression pattern of the lncRNA was increasing during the B16 cell proliferation. Bio-information analysis indicated that LncRNA XLOC_15448 might regulate gene expression of MITF (microphthalmia-associated transcription factor) by targeting to miR-182-5p. It was speculated that there may be a lncRNA-miRNA-mRNA network which modulate the dermal melanogenesis and melanin deposition in Youzhou dark goat, which would provide the basic data for further research on the regulatory mechanisms of lncRNAs related to goat skin pigmentation.
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Received: 28 August 2019
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Corresponding Authors:
*zhangli10078244@163.com
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