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| Tissue Expression of ACACA Gene and Methylation Pattern Analysis of Adipose Tissue in Tianzhu White Yak (Bos grunniens) |
| SHI Xue-Hong1, SHI Bin-Gang1, ZUO Zhi1, ZHAO Shi-Jie1, HE Zhao-Hua1, ZHANG Xue-Ping1, ZHAO Zhi-Dong1, WANG Ji-Qing1, ZHOU Zhi-De2, HU Jiang1,* |
1 Faculty of Animal Science and Technology, Gansu Agricultural University/Gansu Key Laboratory of Herbivorous Animal Biotechnology/Gansu Engineering Lab of Genetic Improvement in Ruminants, Lanzhou 730070, China;
2 Tianzhu County Songshan Town Animal Husbandry Veterinary Station, Tianzhu 733200, China |
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Abstract Acetyl-CoA carboxylases alpha (ACACA) is a key rate-limiting enzyme in the de novo synthesis of fatty acids, which plays an important role in the regulation of fatty acid biosynthesis. In this study, 0.5-year-old and 4.5-year-old Tianzhu white yaks (Bos grunniens) were studied using quantitative real-time PCR (qRT-PCR) and heavy sulfite sequencing (BSP) methods. The expression of ACACA gene in 13 tissues such as heart, liver, and subcutaneous fat, as well as the methylation level of CpG island in PⅠ promoter region in subcutaneous adipose tissue were detected, and the gene expression regular and epigenetic mechanism in subcutaneous adipose tissue were studied. The results showed that the expression of ACACA gene in yaks were different in tissues and ages.The liver, brain, dorsal longest muscle and kidney tissues of 0.5-year-old yaks were highly expressed and the liver expression was significantly higher than other tissues (P<0.05);The expression of subcutaneous adipose and liver in 4.5-year-old yaks was extremely significantly higher than other tissues (P<0.01); The expression in the kidney of 0.5-year-old yaks were extremely significantly higher than that of 4.5-year-old yaks (P<0.01), but the expression of subcutaneous adipose, liver and testicular was significantly or extremely significantly less than that 4.5-year-old yaks (P<0.05 or P<0.01).In the subcutaneous adipose tissue, the ACACA gene PⅠ promoter 1st CpG island area (g.-795 bp~-193 bp) was highly demethylated and there were age differences. The average methylation level of BSP-1 primer amplification zone in 0.5-year-old yaks was extremely significantly higher than that in 4.5-year-old yaks (P<0.01). 0.5-year-old yaks BSP-1 primer amplification zone CpG11, CpG31, CpG35, CpG37 site and BSP-2 primer amplification zone CpG5', CpG13', CpG15', CpG27', CpG28' site methylation level significantly or extremely significantly higher than 4.5-year-old yaks (P<0.05 or P< 0.01); The level of methylation in the 1st CpG island region of the PⅠ promoter was negatively correlated with the expression of the gene in subcutaneous adipose tissue. This study revealed the differences of ACACA gene expression and PⅠ promoter methylation in yaks of different ages, which provide theoretical basis for molecular genetic research of yaks.
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Received: 21 October 2019
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Corresponding Authors:
*, huj@gsau.edu.cn
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