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农业生物技术学报  2019, Vol. 27 Issue (7): 1322-1330    DOI: 10.3969/j.issn.1674-7968.2019.07.020
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Breeding of High Avilamycin-producing Strains by Ribosome Engineering
LIU Hua-Hua, CHEN Yu-Hang, CHEN Min*
School of Food Sciences and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China
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Abstract  Avilamycin is widely used as a new type of metabolic regulator and digestive enhancer in livestock feeding. The low production capacity of the strain and the immature fermentation process limit the industrial of avilamycin in China. Ribosome engineering technique was used to carry out resistance breeding of the Streptomyces viridochromogenes gs 77 by streptomycin to obtain a high-yield mutant strain of avilamycin, . Through the drug sensitive test and high performance liquid chromatography, a high-yield avilamycin mutant S.viridochromogenes gs 77-54 was obtained. The yield of avilamycin reached the maximum at 8th day of shake flask, and increased to 1.80 fold than that of the original strain. Passage experiments showed that the high-yield performance of mutant strains was genetically stable. rpsl gene (encoding the ribosomal protein S12) and the morphological characteristics of the strains parent and after the mutation were analyzed. The results showed that the aerial hyphae of the mutant strain were long and straight, and the spores were elliptical with a diameter of about 1.1 µm× 0.6 µm, which was significantly different from the starting strain. Sequence analysis of the rpsl gene revealed a point mutation in the gene fragment, the G mutation at position 356 was mutated A, which was mutated to glutamine by arginine. The avilamycin high-yield mutant strain was constructed successfully, which would provide a new idea for the breeding of S. viridochromogenes.
Key wordsAvilamycin      Ribosome engineering      Breeding of strains      Streptomycin resistance     
Received: 26 November 2018     
ZTFLH:  S182  
  Q815  
Corresponding Authors: chenmin@zjgsu.edu.cn   
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LIU Hua-Hua
CHEN Yu-Hang
CHEN Min
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LIU Hua-Hua,CHEN Yu-Hang,CHEN Min. Breeding of High Avilamycin-producing Strains by Ribosome Engineering[J]. 农业生物技术学报, 2019, 27(7): 1322-1330.
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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.07.020     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I7/1322
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