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Abstract Abstract: Entomopathogenic nematodes (EPNs) are a class of highly effective and safety biocides whose capacity of reproduction is an important factor affecting the yield and quality of their products. This study aims to identify the biological functions of lin-41 gene in Heterorhabditidoides chongmingensis DZ0503CMFT to clarify the role of this gene in the growth and development of nematodes. According to the differentially expression genes （DEGs）profile of H. chongmingensis nematodes constructed in previous study, the gene lin-41 involved in regulating the development of nematodes was selected. The cloning vector was constructed by using homology analysis and selecting the functional region fragment of lin-41 gene, and the dsRNA expression vector of the fragment was constructed by L4440 plasmid vector to transform into E. coli HT115 (DE3). The expression of the target gene was disturbed in the H. chongmingensis nematodes by feeding with the transformed E. coli HT115 (DE3), and the effect of RNAi and the potential relationship between lin-41 and the reproductive related gene goa-1 were detected by real-time quantitative PCR. The biological characteristics of the H. chongmingensis nematodes after the down-regulation of lin-41 gene expression were recorded. The lin-41 conserved region of H. chongmingensis nematodes contains the NHL superfamily domain, and the dsRNA expression vector constructed for this region can effectively reduce the expression of lin-41 in H. chongmingensis nematodes, which is 72.08% ( P<0.01)lower than that of L4440 group. The number of eggs laid by H. chongmingensis nematodes after lin-41 knocked down was significantly less than that of L4440, whereas no significant change in body length and female rate were detected. In addition, lin-41 RANi significantly reduces the expression of goa-1(P<0.05). The biological function of lin-41was explored by RNAi in H. chongmingensis nematodes, which is involved in regulating the oogenesis of nematodes.
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Received: 15 March 2019
Published: 03 June 2019
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