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Abstract Bothrogonia ferruginea Fabricius is an important agricultural and economic pest in China. Sequencing and analyzing the complete mitochondrial genome of B. ferruginea and exploring its phylogenetic status in the suborder Auchenorrhyncha at the mitochondrial level were of great significance to its systematic taxonomy. The complete mitochondrial genome sequence of B. ferruginea was obtained by PCR amplification and sequencing, and its general features and base composition were analyzed. The plyogenetic tree of mitochondrial protein-coding genes of twenty-two species of Auchenorrhyncha and two outgroups was constructed by using the Bayesian inference (BI) and maximum likelihood method (ML). The complete mitochondrial genome of B. ferruginea is 15262 bp (GenBank accession no: KU167550), including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and one control region. The nucleotide composition and gene distribution sequence of B. ferruginea show the same as insect of Auchenorrhyncha. The A + T content of mitochondrial genome is 76.48%, and the AT-skew is 0.1729. There were 14 overlapping genes and 10 intergenic regions. All PCGs use the ATN as initiation codon. Majority of PCGs have a complete termination codon (TAA or TAG), except that CO II gene terminates with the incomplete stop codon TA. All the predicted tRNAs show the classic clover-leaf structure, except for the absence of DHU arm of tRNASer( AGN). The A + T rich region contains some conserved sequence (ATTTA) and a typical microsatellite structure (TA)25. Two special structures, ATCTA and CCCTCT, were found in the tandem repeats in the control region of Cicadellidae, but not in the family Membracidae and other Auchenorrhyncha. The phylogenetic relationships of 22 species of Auchenorrhyncha based on mitochondrial genome were identified as Cercopoidea + (Fulgoroidea + Membracoidea). B. ferruginea, Nephotettix cincticeps, Idioscopus nitidulus, Empoasca vitis and Homalodisca vitripennis belonged to Auchenorrhyncha, Membracoidea and Cicadellidae. The gene arrangement pattern of the mitochondrial genome of B. ferruginea is identical to those of other Cicadellidae insects. The phylogenetic relationship of Auchenorrhyncha constructed based on the mitochondrial genome is consistent with those in traditional morphological taxonomy. B. ferruginea belonged to Auchenorrhyncha, Membracoidea and Cicadellidae. This paper provides data for the further study of auchenorrhyncha classification system.
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Received: 31 January 2019
Published: 03 June 2019
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