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| Genome Identification and Expression Pattern Analysis of Phyllostachys edulis B3 Family |
| WU Jia-Jun1, YU Shuai-Cheng1, LIU Zhi-Gang1, FU-Ying1, *, ZHOU Ming-Bing1, 2, * |
1 The State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China;
2 Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-efficiency Utilization, Zhejiang A&F University, Hangzhou 311300, China; |
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Abstract The B3 family is a family of transcription factors that contains the B3 domain, which plays an important role in the growth and development of plants. In order to analysis the structure and expression pattern of Phyllostachys edulis B3 family genes, in this study, a total of 78 P. edulis B3 family genes were identified using bioinformatics techniques and methods. These genes could be divided into 4 evolutionary branches of the families, including LEAFY COTYLE-DON2[LEC2]-ABSCISIC ACID INSENSITIVE3[ABI3]-VAL (LAV), auxin response factor (ARF), related ABI3-VP1 (RAV) and reproduction meristem (REM). The transcriptome analysis of 7 materials in moso bamboo showed that the expression patterns of the 78 B3 family genes of could be divided into 5 groups, and B3 family genes had different expression level in 7 materials in moso bamboo. The first 15 genes were mainly expressed in panicles; 20 genes in the second group were mainly expressed in roots; 23 genes in the third group were mainly expressed in young shoots; 7 genes in the fourth group were mainly expressed in leaves; 13 genes in the fifth group were mainly expressed in bamboo rhizome. So, the B3 family of P. edulis plays a important role during the growth of moso bamboo. Two genes were selected from 4 subfamilies to detect the expression patterns of B3 family of P.edulis in different parts of bamboo shoots by qRT-PCR. The results showed that the P. edulis family genes were diversely expressed in different parts of bamboo shoots. Most of B3 family members had high expression in most internode tissues. For example, PH01000024G2370, PH01000297G0830, PH01001094G0220, PH01000114G0050, PH01000548G0300 and PH01000528G0920 had high levels of expression in the 2-4 node internode (internode 2-4). Only 2 genes (PH01000198G0380, PH01001094G0220) had high levels of expression in interval. There were 6 genes (PH01000297G0830, PH01000198G0380, PH01000114G0050, PH01000548G0300, PH01000528G0920 and PH01000469G0440) with high levels of expression in sheath, and one gene (PH01000024G2370) was almost no expression. Only one gene (PH01000024G2370) had a high level of expression in roots. Results suggested that the ARF family (eg PH01000114G0050, PH01000548G0300) played an important role in the rapid growth of bamboo shoots. According to the expression patterns of RAV family (e.g. PH01000024G2370, PH01000297G0830), it was presumed that the growth of shoots in roots and near roots was relatively inhibited during the rapid jointing growth of bamboo shoots. This study systematically investigated the structure, phylogenetic relationship and the expression pattern of B3 family genes of P. edulis, which provides basic data for the further study of B3 family gene function.
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Received: 04 June 2018
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Corresponding Authors:
*, fuying@zafu.edu.cn; zhoumingbing@zafu.edu.cn
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