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| Analysis of Molecular Characteristics and Stress Expression of Carotenoid Hydroxylase Gene Families in Dunaliella viridis |
| YIN Hang, GONG Yi-Fu*, YU Kai, ZHANG Li, CHEN Jun-Yue, WANG He-Yu |
| 1 School of Marine Sciences, Ningbo University, Ningbo 315832, China |
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Abstract Carotene hydroxylases are key enzymes that catalyze the synthesis of carotenoids in metabolic pathway of carotenoids. It consists of the β-carotenoid hydroxylase (chyb) and the cytochrome P450 (cyp97) family. In order to investigate the bioinformatic characteristics of the chyb and the cyp97 gene family and the expression of chyb and cyp97 under different stress conditions in Dunaliella viridis, cDNA sequences of chyb1, chyb2 and cyp97c of Dunaliella viridis were obtained from the transcriptome data. Bioinformatic analysis showed that the proteins encoded by chyb1, chyb2 and cyp97c genes of D. viridis were hydrophilic proteins, of which CHYB1 and CHYB2 had four transmembrane structures respectively, and CYP97C protein had no cross-membrane structure. Multiple sequence alignment showed that both CHYB1 and CHYB2 contained conservative histidine residues (HXXXXH and HXXHH) while CYP97C contained a heme site (FXXGXRXCXG) and a conservative site (EXXR). Phylogenetic tree analysis revealed that the chyb1 and chyb2, belonging to chyb gene family, while cyp97c was a member of cyp97 gene family and most closely related to the Chlamydomonas reinhardtii. Moreover, qRT-PCR results revealed that all of the 3 tested genes were up-regulated (P<0.01) during high light stress. Besides the content of lutein, zeaxanthin, and the active oxygen level in D. viridis increased significantly, indicated that carotenoid hydroxylase gene family was involved in high light stress and regulated reactive oxygen level to protect algal from oxygen stress. In addition, excess salt or low temperature stress or plant growth regulators (e.g. methyl jasmonate, acetylsalicylic acid, arachidonic acid, ammonium cerous sulfate) could not induce these two family genes simultaneously, revealed that different family genes had different preference to stress response. The present study initially revealed the metabolic control mechanism of carotenoid of D. viridis, which provides excellent genetic resources for increasing the content of lutein and zeaxanthin, and a new sight for improving plant stress tolerance.
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Received: 08 April 2018
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Corresponding Authors:
* , gongyifu@163.com
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