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| Isolation and Identification of Yersinia entomophaga Strain Ye1-8 and Its Toxicity to the Red Palm Weevil (Rhynchophorus ferrugineus) |
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Abstract Rhynchophorus ferrugineus is a major quarantine pest that damages the coconut trees (Cocos nucifera) and other palm plants (Arecaceae). In this study, the strain Yel-8 was isolated from the corpse of R. ferrugineus, which had insecticidal activity against the larvae of R. ferrugineus which was suffered from sepsis and identified by morphological identification, 16S rDNA, physiological and biochemical tests, and drug susceptibility analysis, respectively. The results showed that Yel-8 was in line with the characteristic of Yersinia entomophaga in L-arabinose, rhamnose, xylose, sucrose, aesculin, and cellobiose metabolism, whereas opposed to Yersinia only in sorbitol metabolism. Therefore, this strain was identified as a new strain of Yersinia genus. The bioassay of Yel-8 against R. ferrugineus was performed using feeding and injection methods, and the median lethal concentration (LC50) of Yel-8 against R. ferrugineus was 3.184×108 and 5.1295×106 CFU/mg, respectively. The chitinase genes Chi1 and Chi2 were cloned from the Ye1-8 strain and the proteins were expressed for bioassay. The results showed that the Chi1 and Chi2 proteins had no insecticidal activity against R. ferrugineus alone, thus suggesting the necessity of complete toxin complex for toxicity. Yel-8 exhibited insecticidal activity may provide an ideal material for the construction of the biophytes against R. ferrugineus.
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Received: 13 September 2018
Published: 20 November 2018
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