|
|
|
| Expression of MITF Gene in Putian Black Duck (Anas anas domesticus) and Its Association with Melanin Deposition |
|
|
|
|
Abstract Putian black duck (Anas anas domesticus) is the sole native variety in black-feather laying duck, which is rare waterfowl resource in China. The microphthalmia-associated transcription factor (MITF), the most important transcription factor of melanocytes, could be induced by α-MSH (melanocyte-stimulating hormone) and bind to the cAMP responsive element binding protein (CREB) to activate MITF gene expression in melanocytes which plays a key role in the synthesis of melanin. To investigate the relationship between the expression of the MITF gene and regularity of eumelanin deposition in different tissues of Putian black duck, the MITF gene was firstly cloned and analyzed from the skin of Putian black duck with nested PCR and sequenced (GenBank No. MG516571). MITF gene expression was detected in various tissues of Putian black ducks via real-time quantitative PCR, and deposition of eumelanin in the tissues was measured by UV spectrophotometry. The results showed that the length of MITF gene from Putian black duck was 1 341 bp, which contained the complete CDS sequence (12~1 334 bp) and encoded 440 amino acids. The amino acid homology compared with Anas platyrhynchos was 100%, and it was above 95% compared with chicken (Gallus gallus) and quail (Coturnix japonica). The genetic relationship of MITF between Putian black duck and Anas platyrhynchos was the closest, followed with Anser cygnoides, Silky flow, Gallus gallus, and relationship compared with pig (Sus scrofa), sheep (Ovis aries), and other mammals was the farthest. The mRNA expression of MITF gene in Putian black duck skin tissue was the highest in the tissues (skin＞kidney＞gizzard＞liver＞muscle) (P＜0.01). The deposition of eumelanin was basically consistent with the expression of MITF gene in each tissue of Putian black duck. The expression of MITF gene had significant positive correlation with the production of eumelanin in the liver tissues of Putian black duck (P＜0.05). In summary, the expression of MITF gene had tissue specificity in Putian black duck, which might have positive regulation on the deposition of eumelanin and could be used as a candidate gene for the regulation of melanin deposition. The study could provide basic material for the selection of melanin trait in Putian black duck.
|
|
Received: 06 March 2018
Published: 24 October 2018
|
|
|
|
| [1]安键, 汪明, 樊宝良, 等.柔嫩艾美耳球虫马杜拉霉素抗药株与敏感株的mRNA差异显示[J].畜牧兽医学报, 2006, 37(5):480-484
[2]韩吉龙, 岳耀敬, 裴杰, 等.藏羊刺鼠信号蛋白基因(Agouti)的多态性及不同颜色被毛皮肤组织中Agouti与小眼畸形相关转录因子基因(MITF)表达的定量分析[J].农业生物技术学报, 2013, 21(3):338-347
[3]张丽英, 李祥龙, 周荣艳, 等.不同物种Mitf 基因编码区生物信息学分析[J].安徽农业科学, 2011, 39(22):13298-13300
[4]郑嫩珠, 辛清武, 朱志明, 等.白绒乌鸡MITF基因的cDNA克隆、表达及其对黑色素沉积的影响[J].中国农业科学, 2015, 48(18):3711-3718
[5]张瑞娜, 范瑞文, 程志学, 等.CKD5对羊驼皮肤黑色素细胞TYR和MITF mRNA表达的调节[J].畜牧兽医学报, 2011, 42(12):1712-1717
[6]Bauer G L, Praetorius C, Bergsteinsdottir K, et al.The role of MITF phosphorylation sites during coat color and eye development in mice analyzed by bacterial artificial chromosome transgene rescue[J].Genetics, 2009, 183(2):581-594
[7]Dorazio J A, Nobuhisa T, Cui R, et al.Topical drug rescue strategy and skin protection based on the role of Mc1r in UV induced tanning[J].Nature, 2006, 443(7109):340-344
[8]Dong C S, Wang H D, Xue L L, et al.Coat color determination by miR-137 mediated down-regulation of microphthalmia-associated transcription factor in a mouse mode[J].RNA, 2012, 18(9):1679-1686
[9]Goding C R..A picture of Mitf in melanoma immortality[J].Oncogene, 2011, 30(20):2304-2306
[10]Hauswirth R, Haase B, Blatter M, et al.Mutations in MITF and PAX3 cause “Splashed White”and Other White Spotting Phenotypes in Horses[J].Plos Genetics, 2012, 8(4):1-9
[11]Ito S, Nakanishi Y, Valenzuela R.K, et al..Usefulness of alkaline hydrogen peroxide oxidation to analyze eumelanin and pheomelanin in various tissue samples: application to chemical analysis of human hair melanins[J].Pigment Cell Melanoma Research, 2011, 24(4):605-613
[12]Kingo K, Aunin E M, Ratsep R, et al.Expressional changes in the intracellular melanogenesis pathways and their possible role in the pathogenesis of vitiligo[J].Journal of Dermatological Science, 2008, 52(1):39-46
[13]Lin J Y, Fisher D E.Melanocyte biology and skin pigmentation[J].Nature, 2007, 445(7130):843-850
[14]Lee H D, Lee W H, Roh E, et al.Manassantin A inhibits cAMP induced melanin production by down-regulating the gene expressions of MITF and tyrosinase in melanocytes[J].Experimental Dermatology, 2011, 20(9):761-763
[15]Levy C, Fisher D E.Dual roles of lineage restricted transcription factors: The case of MITF in melanocytes[J].Transcription, 2011, 2(1):19-22
[16]Levy C, Khaled M, Fisher D E.Review:MITF:master regulator of melanocyte development and melanoma oncogene[J].Trends in Molecular Medicine, 2006, 12(9):406-414
[17]Loercher A E, Tank E M, Delston R B, et al.MITF links differentiation with cell cycle arrest in melanocytes by transcriptional activation of INK4A[J].Cell Biology, 2005, 168(1):35-40
[18]Li S J, Wang C, Yu W H, et al.Identification of Genes Related to White and Black Plumage Formation by RNA-Seq from White and Black Feather Bulbs in Ducks[J].PLoS ONE, 2012, 7(5):36592-36592
[19]Minvielle F, Bedhom B, Coville J L, et al.The “silver”Japanese quail and the MITF gene:causal mutation, associated traits and homology with the “blue” chicken plumage[J].BMC Genetics, 2010, 11(15):1-7
[20]Nishimura E K, Granter S R, Fisher D E.Mechanisms of hair graying:incomplete melanocyte stem cell maintenance in the niche[J].Science, 2005, 307(5710):720-724
[21]NiC, Zhang D, Beyer LA, et al.Hearing dysfunction in heterozygous Mitf(Mi-wh) /+ mice, a model for Waardenburg syndrome type 2 and Tietz syndrome[J].Pigment Cell Melanoma Res, 2013, 26(1):78-87
[22]Otreba M, Rok J, Buszman E, Wrzesniok D.Regulation of melanogenesis：the role of cAMP and MITF[J].Postepy Higieny i Medycyny Doswiadczalnej, 2012, 66(0):33-40
[23]Ozeki H, Ito S, Wakamatsu K, Thody A J.Spectrophotometric characterization of eumelanin and pheomelanin in hair [J].Pigment Cell Research, 1996, 9(5):265-270
[24]Pfaffl M W.A new mathematical model for relative quantification in realtime RT-PCR [J].Nucleic Acids Research, 2001, 29(9):2004-2007
[25]Shi Y F, Li X Z, Ju D, et al.Anovel mutation of the MITF gene in a family with Waardenburg syndrome type 2:A case report[J].Experimental & Therapeutic Medicine, 2016, 11(4):1516-1518
[26]Sultana H, Seo D, Choi NR, et al.Identification of polymorphisms in MITF and DCT genes and their associations with plumage colors in Asian duck breeds[J].Asian-Australasian Journal of Animal-Sciences, 2018, 31(2):180-188
[27]Takeda K, Hozumi H, Nakai K, et al.Insertion of long interspersed element-1 in the Mitf gene is associated with altered neurobehavior of the black-eyed white(mi-bw)mouse[J].Genes Cells, 2014, 19(2):126-140
[28]Vachtenheim J, Borovansky J.Transcription physiology’’ of pigment formation in melanocytes: central role of MITF[J].Experimental Dermatology, 2010, 19(7):617-627
[29]Wickelgren I.Why I have red hair, need to avoid the sun, and shouldn , and shouldn't commit a crime[J].Science, 2007, 315(5816):1215-1215
[30]Wan P, Hu Y Q, He L.Regulation of melanocyte pivotal transcription factor MITF by some other transcription factors[J].Molecular and Cellular Biochemistry, 2011, 354(1):241-246
[31]Wang Y, Li S M, Huang J, et al.Mutation of TYR and MITF genes with are associated with plumage color phenotypess in geese[J].Asian-Australasian Journal of Animal-Sciences, 2014, 27(6):778-783
[32]Yan X K, Zhang T Y, Wang Z M, et al.A novel mutation in the MITF may be digenic with GJB2 mutations in a large chinese family of Waardenburg syndrome type Ⅱ[J].Journal of Genetic and Genomics, 2011, 38(12):585-591
[33]Zhu Z, He J, Jia X, et al.MicroRNA-25 functions in regulation of pigmentation by targeting the transcription factor MITF in alpaca(Lama pacos) skin melanocytes[J].Domestic Animal Endocrinology, 2010, 38(3):200-209
|
|
|
|
