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| Study on the Function of Zinc Finger Protein Gene EgrZFP7 in Cold Stress Response of Eucalyptus grandis |
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Abstract Abstract Zinc finger proteins (ZFPs) are a class of transcriptional factors that play important roles in growth, development and stress responses in plants. Finding more genes which have key function in cold stress response can give support for cold tolerance breeding in Eucalyptus trees with molecular assisted selection. A zinc finger protein gene induced under treatment at low temperature was screened in Eucalyptus grandis. The gene was named EgrZFP7. EgrZFP7 protein structure and its function were analyzed in the research.This gene is a classical C2H2 type zinc finger protein gene. The protein sequence it encoding contains 2 zinc finger motifs, 1 L-Box motif and 1 EAR motif. EgrZFP7∷GFP vector was constructed and subcellular localization of EgrZFP7 was verified by bombardment of onion epidermis with a gene gun.The result showed that it localized in nuclear. The vector of 35S∷EgrZFP7 was also constructed and transformed into Arabidopsis thaliana. Two homozygoteslines of EgrZFP7-OX1 and EgrZFP7-OX2 were obtained. Under normal growth condition, the 2 EgrZFP7 over-expression lines showed more lateral roots and the length of lateral roots were also longer than that of the wide type. However, when EgrZFP7-OX1 and EgrZFP7-OX2 were treated at -8 ℃ for 3 d, then recovered for 3 d under normal temperature, the speed of growth recovery of over-expression lines was slower than that of the wild type. Finally, death percentage of total seedlings EgrZFP7-OX1 and EgrZFP7-OX2 lines were obviously higher than that of the wild type, implying that over-expression of EgrZFP7 could increase sensitivity to low temperature in seedlings. A protein, EgrERF4 (Eucalyptus grandis ethylene response factor 4), which can interact with EgrZFP7 was screened and verified from Yeast Two Hybrid Library. It could be concluded that EgrZFP7 possibly play a role in gene regulation in cold stress response through interact with EgrERF4 in Eucalyputs grandis. The present study gives more information to learn the mechanism of cold stress response in Eucalyptus.
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Received: 05 December 2017
Published: 20 July 2018
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| [1]Chang H, Chen D, Kam J, et al. 2016. Abiotic stress upregulated TaZFP34 represses the expression of type-B response regulator and SHY2 genes and enhances root to shoot ratio in wheat[J]. Plant Sci, 252, 88(7):88-102 [2]Devaiah B N, Nagarajan V K, Raghothama K G. 2007. Phosphate homeostasis and root development in Arabidopsis are synchronized by the zinc finger transcription factor ZAT6[J]. Plant Physio, 145(1):147-159[3]Englbrecht C C, Schoof H, B?hm S. 2004. Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome[J]. BMC Genomics, 5(1): 1-17[4]Gao S, Zhang H, Tian Y, et al. Expression of TERF1 in rice regulates expression of stress-responsive genes and enhances tolerance to drought and high-salinity[J]. Plant Cell Rep, 2008, 27(11):1787-1795 [5]Joseph M P. 2014. The Arabidopsis Zinc Finger Protein 3 interferes with ABA and light signaling in seed germination and plant development[J]. Plant physiol, 165(3): 1203-1213[6]Kagale S, Rozwadowski K. 2011. EAR motif-mediated transcriptional repression in plants: An underlying mechanism for epigenetic regulation of gene expression[J]. Epigenetics, 6(2): 141-146[7]Kie?bowicz-Matuk A. 2012. Involvement of plant C2H2-type zinc finger transcription factors in stress responses[J]. Plant Sci, 185(4): 78-85[8]Kim J C, Lee S H, Cheong Y H, et al. 2001. A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants[J]. Plant J Cell & Mol Bio, 25(3):247-59 [9]Klug A, Schwabe J W. 1995. Protein motifs 5. Zinc fingers[J]. FASEB J, 9(8): 597-604Kodaira K S, Yamaguchi-Shinozaki K. 2011. Arabidopsis Cys2/His2 zinc-finger proteins AZF1 and AZF2 negatively regulate abscisic acid-repressive and auxin-inducible genes under abiotic stress conditions[J]. Plant Physio, 157(2):742-756 [10]Laity J H, Lee B M, Wright P E. 2001. Zinc finger proteins: new insights into structural and functional diversity[J]. Curr Opin Struct Biol, 11(1): 39-46[11]Liu D, Yang L, Luo M, et al. 2017. Molecular cloning and characterization of PtrZPT2-1, a ZPT2 family gene encoding a Cys2/His2-type zinc finger protein from trifoliate orange ( Poncirus trifoliata (L.) Raf.) that enhances plant tolerance to multiple abiotic stresses[J]. Plant Sci, 263(10): 66-78[12]Mittler R, Kim Y S, Song L, et al. 2006. Gain- and loss-of- function mutations in Zat10 enhance the tolerance of plants to abiotic stress[J]. FEBS Lett, 580(12): 6537-6542[13]Newbigin E, Wan L, Zhang J, et al. 2011. Transcriptional Activation of OsDERF1 in OsERF3 and OsAP2-39 Negatively Modulates Ethylene Synthesis and Drought Tolerance in Rice[J]. Plos One, 6(9): e25216[14]Ohta M, Matsui K, Hiratsu K, et al. 2001. Repression Domains of Class II ERF Transcriptional Repressors Share an Essential Motif for Active Repression[J]. Plant Cell, 13(8): 1959-1968[15]Okamuro J K, Caster B, Villarroel R, et al. 1997. The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis[J]. PNAS, 94(13): 7076-7081Sakamoto H. 2004. Arabidopsis Cys2/His2-Type Zinc-Finger Proteins Function as Transcription Repressors under Drought, Cold, and High-Salinity Stress Conditions[J]. Plant Physio, 136(1): 2734-2746[16]Shigyo M, Hasebe M, Ito M. 2006. Molecular evolution of the AP2 subfamily. Gene, 366(2): 256-265[17]Sugano S, Kaminaka H, Rybka Z, et al. 2003. Stress-responsive zinc finger gene ZPT2-3 plays a role in drought tolerance in petunia[J]. Plant J, 36(6): 830-841[18]Takatsuji H. 1999. Zinc-finger proteins: the classical zinc finger emerges in contemporary plant science[J]. Plant Mol Biol, 39(6): 1073-1078[19]Kamsv?gmagnusson T, Thorsellcederberg J, Svanberg A, et al. 2014. Role of Ethylene Response Transcription Factor (ERF) and Its Regulation in Response to Stress Encountered by Plants[J]. Plant Mol Biol Rep, 33(3): 347-357[20]Wang S, Wei X L, Cheng L J. et al. (2014) Identification of a C2H2-type zinc finger gene family from Eucalyptus grandis and its response to various abiotic stresses[J]. Biol Plantarum 58(2): 385-390[21]Yu Y, Yang D, Zhou S, et al. 2016. The ethylene response factor OsERF109 negatively affects ethylene biosynthesis and drought tolerance in rice[J]. Protoplasma, 254(1): 401-408[22]Zhang H, Zhang J, Quan R, et al. 2013. EAR motif mutation of rice OsERF3 alters the regulation of ethylene biosynthesis and drought tolerance[J]. Planta, 237(6): 1443-1451 |
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