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Association of Polymorphisms in ATP5B Promoter Region with Growth Traits of Nanyang Cattle (Bos taurus) |
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Abstract Abstract The ATP synthase subunit beta (ATP5B) is one of key factors in ATP synthesis, participating in various metabolic activities. Nanyang cattle (Bos taurus) is the one of the five major Chinese native cattle varieties, famous for its adaptability. This study investigated the genetic variation in ATP5B promoter regions and the association of Nanyang cattle growth traits; estimated the conservation of ATP5B among different species, and examined the mRNA expression. It was sequenced the ATP5B promoter region to determine the genetic variants and their association with Nanyang cattle growth traits. To find the reason of this association, It was detected the promoter activity of each genotype promoter at these SNPs site using dual luciferase assay. In the results, there were 14 similar motifs and 5 conservative functional domains in the ATP5B of 7 species, such as, Bos taurus, Sus scrofa, Ruttus and so on. The ATP5B was widely expressed in many tissues in Nanyang cattle, and the muscle tissue had the significant high mRNA level than other tissues (P<0.05), the ATP5B mRNA in fat tissue was following, the next was in heart, kidney, lung, and liver; the ATP5B mRNA in spleen was the lowest compared with other tisuses (P<0.05). 3 SNPs in ATP5B promoter regions were identified, named as g.-428T>A, g.-390T>C and g.-322C>G, respectively. After association analysis, there were 3 mutations fell into Hardy-Weinberg equilibrium significantly (Chi-Squared test, P>0.05). The polymorphism information content (PIC) classification indicated that the 3 SNPs were moderately polymorphic (0.25<PIC<0.50). At g.-428T>A locus, individuals with TT genotype exhibited significantly higher body weight, body height and chest circumference than those with AA genotype (P<0.01). Such as, TA individuals had greater body weight than AA individuals (P<0.01), but not than TT individuals (P>0.05). In body height, the individuals with TT or TA had greater level than AA individuals (P<0.05). In chest circumference, the TT individuals had greater level than the individuals with TA or AA (P<0.01). So, the TT was the preferable genotype. At g.-390T>C locus, the body height and chest circumference of animals with TT genotype were significantly higher (P<0.01) than these traits of animals with CC genotype. TC individuals had greater body height than CC individuals (P<0.05), but less than TT individuals (P<0.01). TC individuals had less chest circumference than TT individuals (P<0.01). So, the TT was the preferable genotype. Moreover, the g.-322C>G locus were significantly affected body weight and body length (P<0.01 or P<0.05), including that, the CC individuals had greater body weight than CG individuals (P<0.05) and GG individuals (P<0.01), meanwhile, CG individuals had greater body weight than GG individuals (P<0.05). In body length, the individuals with CC or CG had greater level than GG individuals (P<0.05). So that, CC was the preferable genotype. Last, according to the report of dual luciferase assay, although there were many difference between each promoter style at each SNP site, but all of them not reached at significant level (P>0.05). The molecular mechanism of regulation between these SNPs and growth traits need to further search. To sum up, these results suggested that the SNPs of ATP5B promoter region, the TT at -428T>A locus, TT at -390T>C locus, and CC at -322C>G locus, affected the growth traits of Nanyang cattle, and they could be used as candidate genotype for breeding programs.
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Received: 19 January 2018
Published: 06 August 2018
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