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Abstract Abstract Detection of quantitative trait loci (QTLs) related to rice quality in different varieties and different environment can provide a basis for breeding and utilization of genes related to excellent cooking and eating quality of rice. The DH population, derived from a cross between CJ06 (Oryza sativa ssp. japonica) and TN1 (Oryza sativa ssp. indica), was used to detect QTLs of grain shape, amylose content (AC), and starch viscosity (rapid viscosity analysis, RVA). Thirteen QTLs related to grain type, amylose content and RVA parameters were obtained by QTL analysis. The QTLs were mapped to chromosome 1, 2, 3, 6, 7, 8, and 10, included qHPV-6, qCPV-6, qBDV-6, qCSV-6, qSBV-1, qSBV-6, qSBV-7, qRL-2, qRLW-2, qRLW-3, qRLW-8-1, qRLW-10, and qAC-6. Seven QTLs on chromosome 1, 6, and 7 that accounted for 7.03% to 49.05% of the total phenotypic variance and their LOD values ranged from 2.55 to 13.18. In these QTLs, contribution rate of different single QTL was more than 25% except qSBV-1 and qSBV-7. Furthermore, qHPV-6, qCPV-6, qBDV-6, qCSV-6, and qSBV-6 were mapped to the same region of chromosome 6, a main effect QTL qAC-6, whose contribution rate was 45.21%, was also detected in the region. Bioinformatics analysis indicated that the region contained a reported Waxy (Wx) gene which regulates amylose synthesis. Five QTLs with additive effects were detected in grain shape. The LOD values ranged from 3.16 to 8.47 and single QTL contribution rate was 8.95% to 27.23%. Three regions of qRLW-2, qRLW-3, and qRLW-8 contained 3 major reported QTLs GW2, GS3 and GW8. The QTL analysis in present study provides basic data for molecular breeding of rice quality.
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Received: 26 December 2017
Published: 06 August 2018
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