Abstract Abstract Bacillus strains are widely used as animal probiotics. Cellulase produced by Bacillus strains could be used to increase efficiency of feed utilization and improve the intestinal microflora. The aim of this study was to screen the Bacillus strains with high celluloytic activities from the rhizosphere soil of plants (sugarcane and hemp, and so on) to provide strain resources for development of animal probiotics. Firstly, culturable Bacillus strains were isolated from the soil samples through dilution seperation method on LB plate. Eight celluloytic Bacillus strains were obtained by screening with the sodium carboxymethyl cellulose (CMC-Na) medium. Among them, the strain FJAT-29941 exhibited the strongest cellulase activities and was identified as Bacillus amyloliquefaciens according to the morphology, physical and biochemical characteristics and 16S rRNA gene sequences analysis. The cellulase production conditions, gastrointestinal tolerance, filter paper degradation and hemolytic activity of strain FJAT-29941 were studied furtherly. The fermentative conditions for cellulase-producing of the strain FJAT-29941 were as follows: pH 4.0~9.0 (optimum pH 9.0), 20~50 ℃ (optimum 30 ℃ ), 18~72 h (optimum 42 h) and the optical fermentative medium was NA (nutrient agar) containing 0.5% NaCl. Moreover, the cellulases displayed excellent tolerance to pH and temperature. The survival rate of the strain FJAT-29941 in the artificial intestinal fluid containing 1% typsin and the artificial gastric fluid containing 1% pepsin was 27.98% and 64.91%, respectively, suggesting a great tolerance to gastrointestinal environments. It was showed that the cultures could rapidly degrade intact long filter paper scrips into fragments within 48 hours of incubation, showing strong ability of degradation on cellulose substrates. Finally, no hemolysis of the strain FJAT-29941 was observed on the goat blood agar plates, indicating its safety toward use of this isolate as a potential probiotics. Taken together, the strain B. amyloliquefaciens FJAT-29941 exhibited both excellent cellulose degrading ability and probiotic potential, which would provide a strain resource and scientific basis for the probiotics development.
LIU Guo-Hong,LIU Bei,CHE Jian-Mei, et al. Screening of a Bacillus amyloliquefaciens Strain and Its Probiotic Characteristics[J]. , 2018, 26(6): 1043-1055.
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