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| Tissue Expression Profile of miR-124a-3p and Its Interaction Relationship with ACAA2 Gene in Chicken (Gallus gallus) |
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Abstract Abstract The miR-124a-3p has a potential interaction relationship with the acetyl-CoA acyltransferase 2 gene (ACAA2) in chicken (Gallus gallus). The research around biological function of chicken miR-124a-3p and its targeting effect on ACAA2 gene have not been reported. This study analyzed the potential biological function of miR-124a-3p by integrating the results of tissue expression profile and target gene prediction, and verified its relationship targeting with ACAA2 in chicken. The results of qRT-PCR analysis showed that miR-124a-3p was expressed both in all the 13 detected tissues at 4 developmental stages, especially high abundance and specific expression in the hypothalamus. Bioinformatics analyses indicated that the predicted target genes of miR-124a-3p were enriched in different biological processes and pathways, such as lipid transport, regulation of neuron migration, adipocytokine signaling pathway and axon guidance. The relevance analysis indicated that the expression of miR-124a-3p and ACAA2 appeared a negative relevance in pectorales, liver and subcutaneous adipose tissue. When miR-124a-3p mimics were transfected in chicken fibroblasts cells, the luciferase activity and expressions of ACAA2 gene were remarkably inhibited. These results demonstrated that miR-124a-3p as a generalized miRNA exhibited striking temporal expression characteristics in chicken, and regulate a variety of biological process related to lipid metabolism by targeting the ACAA2 gene. The data in this study provide a useful clue for further studying the function of miR-124a-3p and uncovering the formation mechanism of traits involved in fat deposition in chicken.
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Received: 04 September 2017
Published: 21 May 2018
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