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| Interaction Between Translationally Controlled Tumer Protein (TCTP) and Thaumatin-Like Protein (TLP) in Wheat (Triticum aestivum) |
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Abstract Abstract Translationally controlled tumor protein (TCTP) exists widely in eukaryotic cells. TCTP was involved in a variety of cellular processes including mitotic regulation, DNA damage repair, and plant resistance to pathogen infection. Thaumatin-like protein (TLP) is a pathogenesis related protein in plants and is thought to participate in the various processes of plants in response to pathogens infection. Previous work in this laboratory has demonstrated that TaTCTP was involved in the response of wheat (Triticum aestivum) to Puccinia triticina infection. This study focused on the interaction between TCTP and TLP. Vectors were constructed for yeast two-hybrid and Bi-molecular fluorescence complementation (BiFC), respectively. Through yeast two-hybrid test, it was found that yeast AH109 carried both TCTP and TLP could grow on SD-Leu/-Trp/-His and SD-Leu/-Trp/-His/-Ade medium and have detectable α- galactosidase gene (MEL1) reporter activity. This result indicated that there is a physical interaction between TaTCTP and TaTLP. Through the BiFC test, it was found that a strong yellow fluorescence signal could be observed in tobacco epidermis cytoplasm when co-transforming Agrobacterium with TCTP and TLP. These results indicated that TaTCTP and TaTLP can interact with each other in cytoplasm. This study lay a foundation for further exploring the function and mechanism of TCTP in wheat resistance to Puccinia triticina infection.
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Received: 08 November 2017
Published: 21 May 2018
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