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| Association Analysis on the SNP of LF -131C>T and LF -28A>C with Milk Performance, Clinical Mastitis and Lifetime for Chinese Holstein(Bos taurus) |
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Abstract Abstract Clinical mastitis is a kind of the most serious disease with high incidence in dairy industry, and has been plagued in the development of dairy industry. It has significant effects on milk performance and lifetime which will cause huge economic losses in milk production process. It's very important to study the related genes about mastitis for the improvement of milking traits and elongation of lifetime of cows with molecular techniques, and scientific basis would be provided for the breeding of anti-disease and improvement of economic benefits of dairy farm. In order to verify the effects of lactoferrin gene polymorphism on the production traits of Chinese Holstein (Bos taurus), SNPs of LF gene were selected by PCR and direct sequencing for 20 cows with low SCS and 20 cows with high SCS according to the sequence of 5' untranslating region (UTR) of LF gene. Finally, LF-131C>T and LF-28A>C for 866 Chinese Holstein cows were detected by flight mass spectrometry. The dairy herd improvement production recordings information including the number of test cow, ID number of test cow's father, date of test, related genealogical information, test-day milk yield, fat content, protein content, somatic cell score, lactose, total solid, milk urea nitrogen, clinical mastitis and productive life of tested cows were collected from the management system of dairy farm. The effects of LF-131C>T and LF-28A>C on milk performances and lifetime were analyzed by multi factor variance analysis and Cox regression. The results showed that the dominant genotype of LF-131C>T and LF-28A>C loci were TT and AA with the frequency of 0.813 and 0.580, respectively. The dominant genes were T and A, respectively, and their frequencies were 0.903 and 0.762, respectively. Two loci were in the state of Hardy-Weinberg Equilibrium. LF-131C>T had extremely significant effects on SCS, lactose content and 305 days milk yield (P＜0.01), the effects on test-day milk yield and protein content were close to significant level (0.05＜P＜0.1), but LF-131C>T did not have significant effects on fat content total solid and milk urea nitrogen (P>0.05). Multiple comparisons showed that individuals with genotype of LF-131CC had significantly higher 305 days milk yield and lower SCS and lactose content than that of LF-131 CT and TT. LF-28A>C extremely significantly affected test-day milk yield, fat content, protein content, lactose content, total solid and 305 days milk yield (P＜0.01), but had no significant effects on SCS and MUN (P>0.05). Individual with LF-28 CC genotype had significantly higher fat content, protein content, total solid and 305 days milk yield and lower lactose content than that of LF-131 AA and AC, they also had significantly higher test-day milk yield than that of LF-131 AA. LF-28A>C had significant effects on production lifetime (P＜0.05)，individuals with CC genotype had shorter lifetime than that of AA genotype. LF -131C>T had effects on lifetime, but the effects were not significant. Relative researches about the association between LF-131C>T and LF-28A>C and clinical had important significance for improving milk performance and prolonging production life of dairy cows. It could provide scientific basis for disease resistance breeding and economic benefit improvement of dairy cows.
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Received: 06 November 2017
Published: 02 May 2018
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