Abstract Abstract Festuca arundinacea is a widely cultivated forage in the world with excellent stress and disease resistance. Qiancao No.2 was cultivated by Guizhou Institute of Prataculture, which has two different strains (broad-leaved and fine-leaved) with obvious differences in the leaf shape. In order to recover the mechanism of leaf formation and resistance of Qiancao No.2, in this study, a differential display reverse transcription PCR (DDRT-PCR) was performed to screen the differential expression genes between the broad-leaved and fine-leaved strains. Finally, 50 differential bands were separated and recovered. The DNA fragments were cloned and sequenced. Most of different expressed genes were enriched into photosynthesis, growth regulation and stress resistance. After further verification by qRT-PCR, it was found that only the genes of 18S ribosome were up-regulated in broad-leaved, and the rest of them were up-regulated in fine-leaved. These results suggested that plants with large leaves usually allocated more biomass to the cell wall while to enhance the toughness of leaves, and accumulated more photosynthetic products to prepare for the growth and development, this situation would lead to lower photosynthetic efficiency in unit area of leaves. However, plants with narrow leaves allocated more organic in chloroplast which made them had higher photosynthetic capacity to accelerate growth, and with strong competition ability in natural environment. In particular, the growth of aboveground parts made them with strong competition ability in natural environment. This study provides a theoretical foundation for the further research on the functional genes of leaf morphogenesis, gene expression and the stress resistance.
XIE Jian,NIU Xi,XU Yao, et al. Study on Differentially Expressed Genes in Leaf of Two Different Festuca Arundinacea Strains[J]. , 2018, 26(5): 793-800.
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