Abstract Genetically modified (GM) soybean (Glycine Max) event SHZD32-1 was developed by Chinese scientists with independent intellectual property rights, and the event has herbicide resistance by translating glyphosate resistant gene G10-EPSPS into cultivated variety 'Zhongdou 32 (Glycine Max)'. At present, GM soybean event SHZD32-1 has entered the stage of environmental testing and has a broad application prospect. So far, there are little reports about detection methods for GM soybean event SHZD32-1. In order to protect and improve the implementation of laws and regulations for GM soybean event SHZD32-1, the specific primer pairs and probes were designed based on the flanking sequences between the soybean genome and inserted exogenous fragment of SHZD32-1. Conventional PCR and qRT-PCR detection methods had been developed in this study. Specificity detection of conventional PCR and qRT-PCR found that all mixture GM samples DNA could not obtain the positive results except containing the SHZD32-1 genomic DNA, indicating the methods were good for specificity. Conventional PCR could detect 0.1% of GM SHZD32-1 event, and limit of detection (LOD) of qRT-PCR method could reach 21 copies of GM SHZD32-1 genomic DNA. The results were obtained by using the conventional PCR and qRT-PCR method for 60 repetitions under the LOD concentration. Therefore, the two methods had high specificity, good sensitivity, and strong stability. This research established GM SHZD32-1 specific PCR detection method is an important content of molecular characteristics of the evaluation of GMO, could be used to GMO safety regulation and provide technical support in China.
YANG Hua,PENG Cheng,XIAO Yang-Beng, et al. Study of Conventional PCR and qRT-PCR Detection Methods for Genetically Modified Soybean (Glycine max) SHZD32-1[J]. , 2018, 26(3): 492-501.
