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| Cloning and Expression Analysis of Waxy-related Genes in Brassica napus |
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Abstract In plants, epicuticular wax plays important roles in plant growth and development, and it is indispensable for plants to adaptation to the external environment. Based on the known protein sequences of wax genes in Arabidopsis thaliana, 44 gene copies for 10 wax inducer 2 (WIN2) gene families were identified and analyzed in the genome reference of rapeseed (Brassica napus) ZS11 by the bioinformatics methods. In this study, the cDNA were isolated from 3 oilseed (L1050, P144 and W481) with the differences on epicuticular waxy of leaves before bolting (BB) and after bolting (AB), respectively. Then they were thoroughly cloned by the specific primers from them using the homology-based cloning strategy. However, homology comparison revealed that the nucleotide and protein had high sequence homology among them, but the SNPs and amino acid mutations were stably detected in the sequences of BnWIN2C01. Meanwhile, the expression levels of BnWIN2C01 were detected using the qRT-PCR among the leaves before bolting (BB) and after bolting (AB) in different B. napus. The results showed that the highly differences were found among them, indicating that the specific expression of BnWIN2C01 might be involved in the biosynthesis of epicuticular waxy. Results will be helpful for deciphering the molecular mechanism of epicuticular waxy in B. napus, and provide a reference to improve the rapeseed stress resistance.
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Received: 24 May 2017
Published: 10 December 2017
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