MC4R基因多态性及基因型组合与藏羊生长性状的关联分析

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Abstract Melanoeortin receptor-4 gene (MC4R) belongs to the super family of G protein-coupled receptors that is implicated in the control of feeding behavior and energy homeostasis in mammals. This study aimed to explore the association of single nucleotide polymorphism (SNP) of MC4R gene with Tibetan sheep (Ovis aries) growth traits, so as to search for molecular markers related with growth traits, DNA sequencing method was applied to detect and genotype polymorphisms within MC4R gene in Tibetan sheep populations, and analyzed the associations of polymorphisms and combined genotypes with growth traits of Tibetan sheep. Results showed that 4 SNPs in MC4R gene were detected, including g.1803G＞A in exon 1, g.1261T＞C in intron 2 and two mutants (g.1803G＞A and g.1918C＞T) in 3'-untranslated region (3'UTR). Association analysis in 555 Tibetan sheep population indicated that, at g.880G＞A locus, individuals with genotype AA had larger higher body weight, body height, body length and chest circumference compared with the genotype GG (P＜0.01 or P＜0.05). At g.1261T＞C locus, the body weight of animals with TC genotype was significantly higher (P＜0.05) than that of animals with TT genotype. Moreover, the g.1803G＞A locus was significantly affected body weight (P＜0.01), and GG was the preferable genotype. The optical combined genotype of MC4R was diplotype H13H7 (GTGC-ATAC), which was more effective on growth traits than other combined genotypes (P＜0.05). These results suggested that the single nucleotide polymorphisms and combined genotypes of MC4R gene affected the growth traits of Tibetan sheep, and they could be used as candidate genes for breeding Tibetan sheep with better growth traits.

Key words： Tibetan sheep Melanoeortin receptor-4 gene (MC4R) Growth traits Molecular marker

Received: 19 April 2017 Published: 14 February 2018

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	DIAO Xian-Lin
	CAO Shao-Jie
	YU Jun-Quan

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DIAO Xian-Lin,CAO Shao-Jie,YU Jun-Quan. Association Analysis of MC4R Gene Polymorphisms and Genotype Combination with Growth Traits of Tibetan Sheep (Ovis aries)[J]. , 2018, 26(3): 429-436.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2018/V26/I3/429

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