Abstract Bovine leukemia is a kind of chronic tumor disease caused by Bovine leukemia virus (BLV) in cattle(Bos taurus), sheep (Ovis aries) and other animals. In order to verify whether there is an association between BLV and SNPs of CXCR1 gene coding sequence (CDS), bovine leukemia infection of 866 Chinese Holstein was detected by fluorescence resonance energy transfer - quantitative polymerase chain reaction (FRET-QPCR) in this study. The SNPs (CXCR1-c.642A>G, CXCR1-c.816C>A, CXCR1-c.980A>G and CXCR1-c.1068G>A) in the CDS of CXCR1 gene were detected by MALDI-TOF-MS (matrix-assisted laser desorption/ ionization time of flight mass spectrometry). The relationship between genotype and haplotype of CXCR1 SNPs and bovine leukemia infection was analyzed by Logistic regression. The results showed: 1) Of the 866 cows, 42 had bovine leukemia, with a positive rate of 4.85%. 2) The predominant genotypes of CXCR1-c.642 A>G, CXCR1-c.816C>A, CXCR1-c.980A>G and CXCR1-c.1068G>A were GG, CC, AA and GG, with frequencies of 0.580, 0.466, 0.60 and 0.658, respectively. The relative risk of leukemia was significantly affected by CXCR1-c.980A>G locus (P=0.016). The individual of GG genotype was 5.04 times suffering from leukemia than that of AA genotype. CXCR1-c.642A>G, CXCR1-c.816C>A, and CXCR1-c.1068G>A have no significant effects on the susceptibility to bovine leukemia. CXCR1-980 A>G can be used as a marker for disease resistance breeding of dairy cows, and selecting AA genotype individuals can help to reduce the susceptibility of bovine leukemia and improve productivity efficiency.
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