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| Estimation of Genomic C Value in Several Species of Salvia |
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Abstract In order to establish the flow cytometry (FCM) method for estimation of Salvia Genomic C-value, fresh leaves of Salvia japonica, S. barrelieri, S. chinensis, S. patens, S. repens were taken as samples. Based on the improved isolation method and DNA reference standards from Oryza sativa subsp. japonica ‘Nipponbare’, Galbraith's、WPS、GPB、Tris-MgCl2、LB01 was used as buffer separately, and ultimately we selected the best results of LB01. In the linear scale measured, the 1C content of S. japonica, S. barrelieri, S. chinensis, S. patens, S. repens was: 0.604, 1.158, 0.634, 0.579 and 0.954 pg, respectively. The results showed that different Salvias with significant different genomic C value, and C value range of similar plants reported in the literature wassimilar. The results provides basic data for further studying on genomics, cytobiology and germplasm resource for Salvia plants.
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Received: 12 March 2017
Published: 30 October 2017
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