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| Study on Insect Resistance of GhPPO1 Gene in Cotton (Gossypium arboretum) by VIGS Technique |
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Abstract Abstract Polyphenol oxidase (PPO) is a class of copper-containing oxidoreductase, and it is resistant to phytophagous insects or pathogens. In order to study the insect resistance of GhPPO1 gene in cotton (Gossypium arboreum), the gene silencing recombinant vector pTRV2-GhPPO1 was constructed, and was transformed into Agrobaoterium tumefaciens GV3101 by virus-mediated gene silencing (VIGS). The cotton plants were fed to 2rd instar larvae of cotton bollworm (Helicoverpa armigera), and the expression of GhPPO1 gene in cotton plants after GhPPO1 gene silencing was obtained by qRT-PCR. The results showed that the expression of GhPPO1 was inhibited by Tobacco rattle virus (TRV) system, and the expression of GhPPO1 gene was only 20% compared with the control group (empty vector pTRV0) (P＜0.05), and the GhPPO1 gene in cotton had a significant silence effect. Compared with cotton bollworm which was fed with common cotton leaf, the weight of the cotton bollworm fed with cotton leaf which was treated by gene silencing increased by 86% after 72 h, which indicated that GhPPO1 was a kind of defense mechanism gene in cotton. The expression of GhPPO1 in the directly induced leaves with cotton bollworm was higher than that of the indirect induced leaves. The results showed that the ability of cotton plants to direct defenses was weakened, and weight of the cotton bollworm increased. It was speculated that GhPPO1 gene might be involved in cotton direct defense response. This study has important significance for the role of GhPPO1 in cotton herbivorous insects, and lays the foundation for the relationship between cotton bollworm and cotton induced defense.
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Received: 12 December 2016
Published: 02 May 2017
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