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| Transcriptomic Analysis of Pandora neoaphidis in Conidial and Hyphal Stages |
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Abstract Abstract Pandora neoaphidis, one of important Entomophthoromycota fungi, is a representative obligated aphid pathogenic fungi that can infect a variety of aphids and are widely distributed. Under suitable conditions, P. neoaphidis can cause a strong epidemic in the aphids in short term and plays a leading role in biological control of aphids. Here, the RNA-seq data of hyphae and primary conidia were carried out using second-generation sequencing platform Illumina Hiseq 2500, and the differentially expressed genes were assessed systematically. Totally, 90 639 contigs and 49 138 unigenes were detected among the RNA-seq data. Results based on Gene Ontology (GO) functional enrichment analysis showed that 49 138 unigenes were annotated to 47 function categories, covering 3 ontologies including molecular function, cellular component and biological processes. Besides, according to reads per kilobase per million of mapped reads(RPKM), a total of 3 555 genes expressed differentially, involving in 2 294 up-regulated genes and 1 261 down-regulated genes. Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis involved 22 metabolic pathways during the conidia stage, of which carotenoid biosynthesis and glycosaminoglycan degradation were significantly up-regulated against the hyphae stage. These basis data would provide a solid foundation for further excavation and analysis of functional genes related to the growth and development of P. neoaphidis.
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Received: 13 January 2017
Published: 06 August 2017
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