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| Cloning and Functional Analysis of VvMIPS Relevant to Temperature Stress in Grape (Vitis vinifera) |
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Abstract Myo-inositol-1-phosphate synthase (MIPS) is a key rate-limiting enzyme in inositol biosynthetic, and plays important roles in plant growth regulation and abiotic stress response. To investigate fuction of MIPS in grape (Vitis vinifera), the full-length cDNA of VvMIPS was cloned from cultivar Zuoyouhong. Sequence analysis showed that the size of VvMIPS was 1 533 bp (GenBank No. 1984676), encoding a protein of 510 amino acids with molecular weight of 56.3 kD, and isoelectric point of 5.37. VvMIPS presented high similarity with MIPS proteins from Glycine max, Medicago sativa and Trifolium pratense. qRT-PCR analysis showed the expression of VvMIPS was the highest in flower buds, stems, and the lowest in root. In addition, VvMIPS was highly induced by low and high temperature stress, and induced by H2S, abscisic acid (ABA) and H2O2. The recombinant plasmid pET28a-VvMIPS was transformed into Escherichia coli BL21, and expression of VvMIPS protein was tested. The results showed that VvMIPS protein could enhance the resistance of E.coli to low and high temperature stress. This study provides the genetic resources for grape cultivars improvement with lower or higher temperature stress tolerance.
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Received: 02 December 2016
Published: 31 March 2017
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| [1]何亚飞, 李霞, 谢寅峰.植物中糖信号及其对逆境调控的研究进展[J].植物生理学报, 2016, 52(3):241-249[2]李希东, 侯丽霞, 刘新, 等.与葡萄基因表达及其低温胁迫响应的关系[J].园艺学报, 2011, 38(6):1052-1062[3]李芳, 汪晓峰.植物中棉子糖系列寡糖代谢及其调控关键酶研究进展[J].西北植物学报, 2008, 28(03):852-859[4]肖培连, 冯睿杰, 侯丽霞, 等.葡萄基因的克隆及表达特性分析[J].植物生理学报, 2015, 51(03):391-398[5]余芳, 邵兴锋, 许凤, 等.果实低温贮藏期间糖代谢变化研究进展[J].果树学报, 2014, 31(1):125-131[6]张广科, 肖培连, 侯丽霞, 等.葡萄基因的克隆及表达特性分析[J].植物生理学报, 2014, 50(6):829-834[7]Chun JA, Jin UH, Lee JW, et al.Isolation and characterization of a myo-inositol-1-phosphate synthase cDNA from developing sesame (Sesamum indicum L) seeds: functional and differential expression,and salt-induced transcription during germination[J].Planta, 2003, 216(5):874-880[8]Cui M, Liang D, Ma F, et al.Molecular cloning and characterization of a cDNA encoding kiwifruit 1-myo- inositol-1-phosphate synthase,a key gene of inositol formation[J].Molecular Biology Reports, 2013, 40(1):697-705[9]Downes CP, Gray A, Lucocq JM.Probing phosphoinositide functions in signaling and membrane trafficking[J].Trends in Cell Biology, 2005, 15(5):259-68[10]Ende WVD, Peshev D.Sugars as antioxidants in plants [M]. Crop Improvement Under Adverse Conditions. 2013: 285-307.[11]Fernandez-Caballero C, Rosales R, Romero I, et al.Unraveling the roles of CBF1,CBF4,and dehydrin 1 genes in the response of table grapes to high CO2 levels and low temperature[J].Journal of Plant Physiology, 2012, 169(7):744-748[12]Garcia MC, Lamattina L.Gasotransmitters are emerging as new guard cell signaling molecules and regulators of leaf gas exchange [J]. [J].Plant Science, 2013 , 201(202):66-73[13]Hong Z, Feibing W, Si Z, et al.A myo-inositol-1-phosphate synthase gene,IbMIPS1,enhances salt and drought tolerance and stem nematode resistance in transgenic sweet potato[J].Plant Biotechnology Journal, 2015, 14(2):592-602[14]Iqbal M, Afzal A, Yaegashi S, et al.A pyramid of loci for partial resistance to Fusarium solani,fsp. glycines,maintains myo-inositol-1-phosphate synthase expression in soybean roots[J].Theoretical and Applied Genetics, 2002, 105(8):1115-1123[15]Joshi R, Ramanarao MV, Baisakh N.Arabidopsis plants constitutively overexpressing a myo-inositol 1-phosphate synthase gene (SaINO1) from the halophyte smooth cordgrass exhibits enhanced level of tolerance to salt stress[J].Plant Physiology and Biochemistry, 2013, 65(6):61-66[16]Kaur H, Verma P, Petla BP, et al.Ectopic expression of the ABA-inducible dehydration-responsive chickpea L-myo-inositol 1-phosphate synthase 2 (CaMIPS2) in Arabidopsis enhances tolerance to salinity and dehydration stress[J].Planta, 2013, 237(1):321-335[17]Knight M R, Knight H.Low-temperature perception leading to gene expression and cold tolerance in higher plants[J].New Phytologist, 2012, 195(4):737-51[18]Kunz S, Pesquet E, Kleczkowski LA.Functional dissection of sugar signals affecting gene expression in Arabidopsis thaliana[J].Plos One, 2014, 9(6):e100312-e100312[19]Li H, Xu Y, Xiao Y, et al.Expression and functional analysis of two genes encoding transcription factors,VpWRKY1 and VpWRKY2,isolated from Chinese wild Vitis pseudoreticulata[J].Planta, 2010, 232(6):1325-37[20]Liu YH, Offler CE, Ruan YL.Regulation of fruit and seed response to heat and drought by sugars as nutrients and signals[J].Frontiers in Plant Science, 2013, 4(4):282-282[21]Ma L, Yang L, Zhao J, et al.Comparative proteomic analysis reveals the role of hydrogen sulfide in the adaptation of the alpine plant Lamiophlomis rotata to altitude gradient in the Northern Tibetan Plateau[J].Planta, 2015, 241(4):887-906[22]Nishizawa A, Yabuta Y, Shigeoka S.Galactinol and raffinose constitute a novel function to protect plants from oxidative damage[J].Plant Physiology, 2008, 147(3):1251-1263[23]Nunes C, Primavesi LF, Patel MK, et al.Inhibition of SnRK1 by metabolites: tissue-dependent effects and cooperative inhibition by glucose 1-phosphate in combination with trehalose 6-phosphate[J].Plant Physiology and Biochemistry, 2012, 63(4):89-98[24]Odom AR, Stahlberg A, Wente SR, et al.A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control[J].Science, 2000, 287(5460):2026-2029[25]Peng S, Zhu Z, Zhao K, et al.A Novel heat shock transcription factor,VpHsf1,from chinese wild Vitis pseudoreticulata,is involved in biotic and abiotic stresses[J].Plant Molecular Biology Reporter, 2013, 31(1):240-247[26]Pillet J, Egert A, Pieri P, et al.VvGOLS1 and VvHsfA2 are involved in the heat stress responses in grapevine berries[J].Plant and Cell Physiology, 2012, 53(10):1776-92[27]Rolland F, Baenagonzalez E, Sheen J.Sugar sensing and signaling in plants: conserved and novel mechanisms[J].Plant Biology, 2006, 57(57):675-709[28]Shen X, Xiao H, Ranallo R, et al.Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates[J].Science, 2003, 299(5603):112-114[29]Sicher R.Carbon partitioning and the impact of starch deficiency on the initial response of Arabidopsis to chilling temperatures[J].Plant Science, 2011, 181(2):167-76[30]Smart CC, Fleming AJ.A plant gene with homology to D-myo-inositol-3-phosphate synthase is rapidly and spatially up-regulated during an abscisic-acid-induced morphogenic response in Spirodela polyrrhiza[J].Plant Journal, 1993, 4(2):279-293[31]Taji T, Ohsumi C, Iuchi S, et al.Important roles of drought-and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana[J].Plant Journal, 2002, 29(4):417-426[32]Tan J, Wang C, Xiang B, et al.Hydrogen peroxide and nitric oxide mediated cold-and dehydration-induced myo-inositol phosphate synthase that confers multiple resistances to abiotic stresses[J].Plant Cell and Environment, 2012, 36(2):288-299[33]Zhong L, Yang SZ, Long WB, et al.Hydrogen sulfide may be a novel downstream signal molecule in nitric oxide-induced heat tolerance of maize (Zea mays L) seedlings[J].Plant Cell and Environment, 2013, 36(8):1564-72[34]Zhu Z, Shi J, Xu W, et al.Three ERF transcription factors from Chinese wild grapevine Vitis pseudoreticulata participate in different biotic and abiotic stress-responsive pathways[J].Journal of Plant Physiology, 2013, 170(10):923-933 |
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