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| cDNA Coding Region Cloning and Sequence Analysing of Sika Deer (Cervus nippon) YWHAE Gene |
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Abstract Abstract Assignment of the human 14-3-3 epsilon isoform (YWHAE) gene encodes 14-3-3ε protein, which highly specifically expresses during antler development of sika deer(Cervus nippon). The study was to clone the YWHAE gene's cDNA coding domain sequence of sika deer and predict its structure, function and expression profiles. Using antlerogenic periosteum as materials, the sika deer's YWHAE CDS was cloned by RT-PCR and its protein structure and functions were predicted through several bioinformational tools as well. The results showed that the sika deer's YWHAE CDS consisted of 768 base pair (GenBank No. KY797653) that encoded 255 amino acids residues, and the amino acid homology of deer YWHAE shared 99.6% identity with bovine (Bos taurus). The analysis of amino acid sequence revealed that the sika deer YWHAE encoded 14-3-3 protein and its relative molecular weight was 29 163.86 D. Subcellular location of 14-3-3 protein was primarily in the cytoplasm, and it belonged to the water-soluble protein. It was predicted that the 14-3-3 protein contained 19 phosphorylation sites, and there was no glycosylation sites. The secondary structure of the protein mainly had helixs, while the tertiary structure of domain area showed a helical state. The study predicts the characteristics of the 14-3-3 protein structure and provides basis theory on exploring the 14-3-3 proteins interacting with other proteins. Furthermore, it provides a basis for further studying on the regulation mechanism of antler development.
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Received: 09 February 2017
Published: 02 May 2017
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