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Lipid-lowering Function and the Mechanism of Malus xiaojinensis Polyphenols on Mice(Mus musculus) with High-fat Diet |
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Abstract The study was to investigate the lipid-lowering and anti-obesity function and mechanism of Malus xiaojinensis polyphenols (MXP). Hyperlipemia mice (Mus musculus) model was established by feeding high-fat diet and MXP gavage administration (50 and 100 mg/kg) for 7 weeks. The levels of triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) and high density lipoprotein-cholesterol (HDL-C) in serum, the TG and TC in liver, the liver and spleen index were measured. At the same time, the transcription of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and the morphology of liver tissue were detected. Finally, the expression of related proteins (IRS, Akt) in the pathway of glucose and lipid metabolism was detected. The results showed that MXP could obviously resist mice body weight quick increase, MXP could significantly reduce the levels of TG, TC and LDL-C in the serum, TG and TC in liver, and increased the level of HDL-C in mice. MXP also could reduce the inflammatory cytokines including IL-6 and TNF-α and liver injury. Furthermore, MXP could improve the expression of IR and IRS significantly. The MXP seemed to improve the utilization rate of glucose and reduce the levels of inflammatory cytokines in liver, it has the potential to develop into a functional food and health care product. This study provides theoretical basis for scientific and rational development of M. xiaojinensis.
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Received: 31 July 2016
Published: 24 December 2016
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[1]成明昊, 杨晓红, 曾维光, 施强.苹果属新种——小金海棠的研究[J]. 农业科技通讯, 1987, 09:19-20.[J].农业科技通讯, 1987, 09(09):19-20
[2]李楠.海棠多酚的种类及其体外抗氧化活性和抑菌活性研究[D].西北农林科技大学, 2014.
[3]Song H, Zheng Z, Wu J, Lai J, Chu Q, Zheng X.White Pitaya (Hylocereus undatus) Juice Attenuates Insulin Resistance and Hepatic Steatosis in Diet-Induced Obese Mice.[J]. PloS one 2016, 11(2):e0149670.[J].PloS one, 2016, 11(2):e0149670-e0149670
[4]Hasibur R, Yasodha K, Khujista H, et al.Green Tea Polyphenols Stimulate Mitochondrial Biogenesis and Improve Renal Function after Chronic Cyclosporin A Treatment in Rats[J].Plos One, 2013, 8(6):e65029-e65029
[5]An-Na L, Sha L, Yu-Jie Z, et al.Resources and Biological Activities of Natural Polyphenols[J].Nutrients, 2014, 6(12):6020-6047
[6]Tjelle T E, Holtung L, B?hn S K, et al.Polyphenol-rich juices reduce blood pressure measures in a randomised controlled trial in high normal and hypertensive volunteers[J].British Journal of Nutrition, 2015, 114(7):1-10
[7] 王勇.原花青素对正常与肥胖小鼠的脂解作用及其机制研究[D].西北农林科技大学, 2011.
[8]Xiuli B, Wei L, Xichun X, et al.Effects of Callistephus chinensis flower polyphones on improving metabolic disorders in high-fat diet-induced mice[J].Food & Function, 2014, 5(12):3304-10
[9]Dharmarajan S K, Arumugam K M.Comparative evaluation of flavone from Mucuna pruriens and coumarin from Ionidium suffruticosum for hypolipidemic activity in rats fed with high fat diet[J].Lipids in Health & Disease, 2012, 11(7):105-118
[10]Ji W, Guo L, Lian J, et al.Hypolipidaemic mechanisms of action of CM108 (a flavone derivative) in hyperlipidaemic rats[J].Journal of Pharmacy & Pharmacology, 2008, 60(9):1207-1212
[11]Guo L, Hu W R, Lian J H, et al.Anti-hyperlipidemic properties of CM108 (a flavone derivative) in vitro and in vivo[J].European Journal of Pharmacology, 2006, 551(1-3):80-86
[12]Cheng W, Li J T, Hu C.Anti-inflammatory and immunomodulatory activities of the extracts from the inflorescence of Chrysanthemum indicum Linné[J].Journal of Ethnopharmacology, 2005, 101(1-3):334-337
[13]Lu M, Wan M, Leavens K F, et al.Insulin regulates liver metabolism in vivo in the absence of hepatic Akt and Foxo1[J].Nature Medicine, 2012, 18(3):388-95
[14]Ying L, Chang L, Mei-Li L, et al.Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model[J].British Journal of Ophthalmology, 2015, 8(8):13136-44
[15]张力平, 孙长霞, 李俊清, 等.植物多酚的研究现状及发展前景[J].林业科学, 2005, 41(6):157-162
[16]宋立江, 狄莹, 石碧.植物多酚研究与利用的意义及发展趋势[J].化学进展, 2000, 12(02):161-170
[17]高光伟, 冯向东.加味川芎都梁丸对高脂大鼠的药理作用[J].中国当代医药, 2009, 16(7):89-90
[18]Irwin D C, Garat C V, Crossno J T, et al.Obesity-related pulmonary arterial hypertension in rats correlates with increased circulating inflammatory cytokines and lipids and with oxidant damage in the arterial wall but not with hypoxia[J].Pulmonary Circulation, 2014, 4(4):821-831
[19] Ivo Batisti?, Jadranka Stojanovski.Lipids, inflammatory cytokines and immunological[J].Hrvatska Znanstvena Bibliografija I Mzos-svibor, 2012, 2(2):765-766
[20]features of atherosclerosis[J].Hrvatska Znanstvena Bibliografija I Mzos-svibor, [J].Nutrition and Immunology. Humana Press,, 2000., 1(1):203-220
[21] Hennig B, Toborek M, Boissonneault G A.Lipids, Inflammatory Cytokines, and Endothelial Cell Injury[M]// Nutrition and Immunology. Humana Press, 2000:203-220.
[22]魏文茂, 朱丽丽, 罗华丽, 等.双葛解酲丸对酒精性脂肪肝大鼠炎症及脂质代谢的影响[J].第三军医大学学报, 2012, 34(7):609-612
[23]Tahir M, Rehman M U, Lateef A, et al.Diosmin protects against ethanol-induced hepatic injury via alleviation of inflammation and regulation of TNF-α and NF-κB activation[J].Alcohol, 2013, 47(2):1366-1372
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